Nitrotoluene

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Basic data given: comparable to guidelines/standards

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1986

Reference 2

Reference Type:

secondary source

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

4-nitrotoluene

EC Number:

202-808-0

EC Name:

4-nitrotoluene

Cas Number:

99-99-0

Molecular formula:

C7H7NO2

IUPAC Name:

1-methyl-4-nitrobenzene

Details on test material:

- Name of test material (as cited in study report): 4-nitrotoluene
- Analytical purity: not reported

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

Activated sludge was derived from predominantly domestic sewage. The sludge was washed with distilled water.

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

3 h

Test conditions

Details on test conditions:

For exposure synthetic sewage feed and an adequate amount of the stock solution were mixed and filled up to 300 mL with distilled water. 200 mL of activated sludge were added. The mix was aerated at a rate of 1 L/min for 3 hours at 20 °C. After aeration the oxygen consumption was measured for 10 min.

Results and discussion

Effect concentrations

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate