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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Skin irritation / corrosion

Currently viewing:

Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Published, public domain data.

Data source

Reference
Reference Type:
publication
Title:
Data on the toxicologic characteristics of the single, brief effect of ammonia on animals.
Author:
Prokop'eva, A.S., Yushkov, G.G. and Ubasheev, I.O.
Year:
1973
Bibliographic source:
GIG TR PROF ZABOL; 17 (6). 1973 56-57

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Assessment of skin irritation in the rat in vivo following exposure to the gaseous substance
GLP compliance:
no
Remarks:
: older, published study

Test material

Constituent 1
Chemical structure
Reference substance name:
Ammonia, anhydrous
EC Number:
231-635-3
EC Name:
Ammonia, anhydrous
Cas Number:
7664-41-7
Molecular formula:
H3N
IUPAC Name:
ammonia
Details on test material:
No further details

Test animals

Species:
rat
Strain:
not specified
Details on test animals or test system and environmental conditions:
The test animal is a white rat. No other details are given.

Test system

Type of coverage:
not specified
Preparation of test site:
not specified
Vehicle:
unchanged (no vehicle)
Controls:
no
Amount / concentration applied:
The concentration was varied. Visible signs of pathology appeared only at an ammonia concentration of 20-30 mg/L and higher.
Duration of treatment / exposure:
The length of time the skin of the animal was exposed to ammonia was varied. The time length ranged from 5-60 mins.
Observation period:
The observation period was the duration of the exposure therefore between 5-60 mins.
Number of animals:
The number of animals is not stated.
Details on study design:
Ammonia was applied to the skin of rats for 5-60 mins. The effects of application were noted.

Results and discussion

In vivo

Results
Remarks on result:
other: The visible signs of skin pathology appeared only at an ammonia concentration of 20-30 mg/L and higher, mainly in the form of a burn around the anus and in the region of the external genitalia.
Irritant / corrosive response data:
The visible signs of skin pathology appeared only at an ammonia concentration of 20-30 mg/L and higher, mainly in the form of a burn around the anus and in the region of the external genitalia.
Other effects:
No further effects

Any other information on results incl. tables

The measure of skin corrosion was determined by using the LC50, which is tabulated below. The visible signs of skin pathology appeared only at an ammonia concentration of 20-30 mg/L and higher, mainly in the form of a burn around the anus and in the region of the external genitalia.

 

Time length (mins)

LC50 (mg/L)

1

5

 Unable to obtain

2

15

112±4.75

3

30

71.9±52

4

60

48.4±5.84

Applicant's summary and conclusion

Interpretation of results:
corrosive
Remarks:
Criteria used for interpretation of results: EU
Conclusions:
The visible signs of skin pathology appeared only at an ammonia concentration of 20-30 mg/L and higher, mainly in the form of a burn around the anus and in the region of the external genitalia.
Executive summary:

The visible signs of skin pathology appeared only at an ammonia concentration of 20-30 mg/L and higher, mainly in the form of a burn around the anus and in the region of the external genitalia. The LC50 was 112±4.75 mg/L after 15 mins of exposure.