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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1958
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study report meets basic scientific principles

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1958
Report date:
1958
Reference Type:
other company data
Title:
Unnamed
Year:
2002
Report date:
2002

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
Standardised study to determine the acute toxicity of the test item in mice after single i.p. application (BASF-Test).
GLP compliance:
no
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
bis(6-aminohexyl)amine; hexane-1,6-diamine
EC Number:
907-605-7
Cas Number:
68815-47-4
Molecular formula:
C6H16N2 (HMD) C12H29N3 (BHT)
IUPAC Name:
bis(6-aminohexyl)amine; hexane-1,6-diamine
Details on test material:
- Name of test material (as cited in study report): Hexamethylendiamin-Rueckstande

Test animals

Species:
mouse
Strain:
not specified
Sex:
not specified

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
water
Doses:
15.9 - 1260 mg/kg bw
No. of animals per sex per dose:
2-5 animals of unknown sex per dose
Control animals:
no
Details on study design:
- Duration of observation period following administration: 7 days

Results and discussion

Effect levels
Sex:
not specified
Dose descriptor:
LD50
Effect level:
ca. 60 mg/kg bw
Based on:
test mat.
Mortality:
15.9 mg/kg bw: 0/5 animals died
20 mg/kg bw: 0/5 animals died
32 mg/kg bw: 0/5 animals died
50 mg/kg bw: 1/5 animals died within 7 d, thereof none within 48 h
79 mg/kg bw: 5/5 animals died within 7 d, thereof four within 24 h
316 mg/kg bw: 2/2 animals died within 3 min
1260 mg/kg bw: 2/2 animals died within 6 min
Clinical signs:
<= 50 mg/kg bw: no clinical signs observed
79 mg/kg bw: abdominal position, accelerated respiration after 3-5 min and before exitus; additionally, ruffled fur was observed before exitus in the animal that died late
316 mg/kg bw: slight cramps and abdominal position after 3 min and before exitus
1260 mg/kg bw: staggering, abdominal/ lateral position and intermittent respiration after 1-2 min and before exitus
Gross pathology:
(performed in 6 animals)
2 animals that died (1260 mg/kg bw): nothing abnormal observed
4 surviving animals: pale liver in two animals

Applicant's summary and conclusion

Conclusions:
Under the conditions of this test the median lethal dose (LD50) after intraperitoneal injection of the test item was identified to be approximately 60 mg/kg bw in mice.