Phosphorus trichloride

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP, guideline comparable study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1977

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Principles of method if other than guideline:

Mutation screening in S. typhimurium and S. cerevisae

GLP compliance:

no

Remarks:

: study pre-dates GLP

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Phosphorus trichloride

EC Number:

231-749-3

EC Name:

Phosphorus trichloride

Cas Number:

7719-12-2

Molecular formula:

Cl3P

IUPAC Name:

phosphorus trichloride

Constituent 2

Reference substance name:

MCTR-190-77

IUPAC Name:

MCTR-190-77

Details on test material:

The test material is described as a colourless liquid

Method

Target gene:

Reversion to histidine independence (S. typhimurium)

Species / strain

Species / strain 1

Species / strain / cell type:

other: S. typhiumurium TA98, TA100, TA1535, TA1575, TA1538

Details on mammalian cell type (if applicable):

Not applicable

Additional strain / cell type characteristics:

not specified

Species / strain 2

Species / strain / cell type:

Saccharomyces cerevisiae

Details on mammalian cell type (if applicable):

Not applicable

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

Male Sprague-Dawley rat Arochlor 1254-induced liver S9 fraction

Test concentrations with justification for top dose:

The substance was tested in a range of concentrations ( 0.1-500 µl per plate), dissolved in distilled water.

Vehicle / solvent:

water or DMSO (both known to be nonmutagenic)

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

Remarks:

distilled water

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: For non activation assays the following substances were used as positive controls; Methylnitrosoguanidine, 2-Nitrofluorene (NF) and Quinacrine mustard (QM). For the activation assays; 2-Anthramine, 2-Acetylaminofluorene and 9-Aminoquinoline

Details on test system and experimental conditions:

The plates were incubated for 48 hours at 37 deg C.

Evaluation criteria:

The number of colonies on each plate were counted and recorded.

Statistics:

None stated

Results and discussion

Test results

Test results 1

Species / strain:

other: S. typhimurium TA98, TA100, TA1535, TA1537, TA1538

Metabolic activation:

with and without

Genotoxicity:

negative

Remarks:

All tests conducted with/without a metabolic system were negative.

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

The compound was toxic to the strains TA-1535, TA-1537, TA-1538 and TA-100 at 5 µL per plate.

Vehicle controls validity:

valid

Untreated negative controls validity:

not applicable

Positive controls validity:

valid

Test results 2

Species / strain:

Saccharomyces cerevisiae

Metabolic activation:

with and without

Genotoxicity:

negative

Remarks:

All tests conducted with/without a metabolic system were negative.

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

Slight toxicity was observed at 5µL per plate with the strain D4.

Vehicle controls validity:

valid

Untreated negative controls validity:

not applicable

Positive controls validity:

valid

Additional information on results:

No further information

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

The test compound was examined for mutagenic activity in a series of in vitro microbial assays employing Salmonella and Saccharomyces indicator organisms. The compound was tested directly and in the presence of liver microsomal enzyme preparations from Aroclor induced rats. The compound was tested over series of concentrations. The compound was toxic to the strains TA-1535, TA-1537, TA-1538 and TA-100 at concentration of 5µL per plate and higher. Slight toxicity was observed at 5 µL per plate with the strain D4. All the tests conducted with/without a metabolic system were negative for mutagenic activity. In conclusion, the test compound did not demonstrate any mutagenic activity in any of the assays conducted.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

The test compound did not demonstrate any mutagenic activity in any of the assays conducted

Executive summary:

The test substance was investigated for mutagencity in Salmonella typhimurium strains TA98, TA100, TA1535, TA1537 and TA1538; and in Saccharomyces cerevisae strain D4. Assays were performed using five concentrations of between 0.001 -5 ug/plate in the presence and absence of an exogenous metabolic acitvation system (Arochlor 1254 -induced male Sprague-Dawley rat liver S9 fraction). No evidence of mutagenicity was seen under the conditions of this study; toxicity was seen at the higher concentrations of the test material. Positive control compounds (MNNG, 2 -nitrofluorene, quinacrine mustard, 2 -anthramine, 2 -AAF and 8 -aminoquinoline) confirmed the sensitivity of the assay and the efficacy of the S9 fraction.