Registration Dossier
Registration Dossier
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EC number: 218-760-9 | CAS number: 2226-96-2
Additional toxicological data
Administrative data
- Endpoint:
- additional toxicological information
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Original reference not available
Data source
Reference
- Reference Type:
- publication
- Title:
- Antiproliferative effect of piperidine nitroxide TEMPOL on neoplastic and nonneoplastic mammalian cell lines
- Author:
- Gariboldi, M.B., Lucchi, S., Caseri, C., Supino, R., Oliva, C., Monti, E.
- Year:
- 1 998
- Bibliographic source:
- Free Rad. Biol. Med., 24, 913-923
Materials and methods
- Type of study / information:
- antiproliferative activity
Test material
- Reference substance name:
- 4-hydroxy-2,2,6,6-tetramethylpiperidinoxyl
- EC Number:
- 218-760-9
- EC Name:
- 4-hydroxy-2,2,6,6-tetramethylpiperidinoxyl
- Cas Number:
- 2226-96-2
- Molecular formula:
- C9H18NO2•
- IUPAC Name:
- 1-λ1-Oxidanyl-2,2,6,6-tetramethylpiperidin-4-ol
Constituent 1
Results and discussion
Any other information on results incl. tables
4-Hydroxy TEMPO was significantly more toxic to tumour cells than to the corresponding normal cells. No difference in cytotoxicity was observed between cell lines with a multidrug resistant phenotype and the corresponding parental cell lines or between estrogen sensitive and insensitive cell lines. After 2 h exposure no cytotoxic effect was observed, after 24 h irreversible cell damage occurred, that increased with increasing exposure time. The hydroxylamine was significantly less cytotoxic. The cytotoxic action was inhibited by co-exposure to acetylcysteine. Exposure to TEMPOL for 24 h induces a dose dependent accumulation of cells in the G1 phase, upon longer exposure times an increase in G2/M cells is observed. After 24 h exposure to 2.5 mM TEMPOL no detectable DNA degeneration was observed in HBL-100 cells, whereas in MCF-7WT cells internucleosomal DNA fragmentation indicative of apoptosis was observed.
Applicant's summary and conclusion
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