Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 201-603-3 | CAS number: 85-41-6
Specific investigations: other studies
Administrative data
- Endpoint:
- cytotoxicity
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Evaluation of the V79 cell metabolic co-operation assay as a screen in vitro for developmental toxicants
- Author:
- Toraason M, Bohrman JS, and Krieg E
- Year:
- 1 992
- Bibliographic source:
- Toxic. in Vitro 6, 165-174
Materials and methods
- Principles of method if other than guideline:
- Phthalimide was tested for its effect on intercellular communication in the V79 cell metabolic co-operation assay (as inhibition of intercellular communication is proposed to be one of several possible mechanisms of teratogenesis, the V79 cell metabolic co-operation assay is evaluated as a in vitro screen for developmental toxicants).
- GLP compliance:
- not specified
- Type of method:
- in vitro
Test material
- Reference substance name:
- Phthalimide
- EC Number:
- 201-603-3
- EC Name:
- Phthalimide
- Cas Number:
- 85-41-6
- Molecular formula:
- C8H5NO2
- IUPAC Name:
- 1H-isoindole-1,3(2H)-dione
- Details on test material:
- - Name of test material (as cited in study report): Phthalimide
- Analytical purity: no data
Constituent 1
Test animals
- Species:
- other: V79 cell
Administration / exposure
- Vehicle:
- DMSO
- Analytical verification of doses or concentrations:
- no
Doses / concentrations
- Remarks:
- Doses / Concentrations:
25, 50, 100, 150, and 200 µg/ml
Basis:
nominal conc.
- Control animals:
- other: not applicable
Examinations
- Positive control:
- the positive control 12-O-tetradecanoylphorbol-13-acetate (TPA) at 4ng/mL
Results and discussion
- Details on results:
- No cytotoxic effects of phthalimide were observed at any concentration. Phthalimide did not inhibit metabolic co-operation of the V79 cells.
Any other information on results incl. tables
Compound Cytotoxicity metabolic
concentration assay cooperation
assay
[µg/ml]
------------------------------------------------------------------
DMSO 100
± 12 19
± 5
TPA 102
± 14 106
± 11
25 96
± 8 22
± 4
50 104
± 10 23
± 5
100 96
± 8 23
± 6
150 100
± 10 24
± 5
200 99
± 5 22
± 6
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
