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Description of key information

The NOEC of the test item (Glycerides, C16-18 mono-, di-, and tri-, hydrogenated, citrates, potassium salts) is 1000 mg/L.
The test item is not toxic at the limit concentration of 1000 mg/L to activated sludge of a municipal sewage treatment plant.

Key value for chemical safety assessment

Additional information

A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was carried out with the test item (Glycerides, C16-18 mono-, di-, and tri-, hydrogenated, citrates, potassium salts) (batch number: 105618) from 2012-06-11 to 2012-06-12, with the definitive experimental phase on 2012-06-12 at the Dr. U. Noack-Laboratorien, 31157 Sarstedt, Germany. Test system was activated sludge of the municipal treatment plant of 31137 Hildesheim, Germany. The test was carried out under static conditions with the limit test item concentration of 1000 mg/L. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The mean inhibition of respiration for the test item replicates ranged from -16 % to -22 %.

In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item and the reference toxicity was determined. The EC50-value for the reference item was 99.1 mg/L.

The NOEC of the test item (Glycerides, C16-18 mono-, di-, and tri-, hydrogenated, citrates, potassium salts) is 1000 mg/L.

The test item is not toxic at the limit concentration of 1000 mg/L to activated sludge of a municipal sewage treatment plant.