p-phenylbenzaldehyde

Endpoint summary

Key value for chemical safety assessment

Genetic toxicity in vitro

Description of key information

Ames: The test substance is non mutagenic in the bacterial reverse mutation test, both in the presence and absence of a metabolizing system (S9-mix).

Link to relevant study records

Reference

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1983-03-10 to 1983-04-11

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No GLP mentioned. Basic data are given in the study report summary.

Qualifier:

equivalent or similar to guideline

Guideline:

other: Standard Japanese test methods (in according with the Industrial Safety and Health Act)

Deviations:

not specified

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Species / strain / cell type:

S. typhimurium, other: TA 98, TA100, TA 1535, TA 1537; TA1538 and E. Coli WP2uvrA

Metabolic activation:

with and without

Metabolic activation system:

S9 mix

Test concentrations with justification for top dose:

Six doses: 1 - 500 µg/plate or 0.5 - 100 µg/plate
The tests of TA 98 without S9-mix in Salmonella typhimurium were conducted in a concentration of 0.1 - 50 µg/plate.

Vehicle / solvent:

No data

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

not specified

True negative controls:

not specified

Positive controls:

not specified

Details on test system and experimental conditions:

No data

Evaluation criteria:

No data

Statistics:

No data

Species / strain:

S. typhimurium, other: TA 98, TA100, TA 1535, TA 1537; TA1538 and E. Coli WP2uvrA

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

Regarding the above concentration, the growth inhibition of Salmonella typhimurium was confirmed.

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

not specified

Remarks on result:

other: all strains/cell types tested

Conclusions:

Interpretation of results (migrated information):
negative

The test substance is non mutagenic in the bacterial reverse mutation test, both in the presence and absence of a metabolizing system (S9-mix).

Executive summary:

The test substance had been tested for reverse mutation in Salmonella typhimurium TA 98, TA100, TA 1535, TA 1537; TA1538 and Escherichia coli WP2uvrA with and without exogenous metabolic activation at six doses: 1 – 500 µg/plate or 0.5 – 100 µg/plate. In addition, the tests of TA 98 without S9-mix in Salmonella typhimurium were conducted in a concentration of 0.1 – 50 µg/plate. The tests were negative, both in the presence and absence of a metabolizing system (S9-mix).

 

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (negative)

Additional information

Bacterial reverse mutation test

 

The test substance has been tested for reverse mutation in Salmonella typhimurium TA 98, TA100, TA 1535, TA 1537; TA1538 and Escherichia coli WP2uvrA with and without exogenous metabolic activation at six doses: 1 - 500 µg/plate or 0.5-100µg/plate. In addition, the tests of TA 98 without S9-mix in Salmonella typhimurium were conducted in a concentration of 0.1 – 50 µg/plate. The tests were negative, both in the presence and absence of a metabolizing system (S9-mix).

 

Justification for selection of genetic toxicity endpoint

Available study report summary.

Justification for classification or non-classification

Based on the result of the bacterial reverse mutation test to the test substance, classification is not warranted according to the criteria of Regulation (EC) No 1272/2008 (CLP).