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EC number: 236-852-7
CAS number: 13515-40-7
In the acute immobilisation test with Daphnia magna (STRAUS) the effect
of the saturated solution of the submission substance
92/69/EC Method C.2 (1992) compliant to GLP (reliability category 1).
The limit test was conducted under static conditions over 48 h. 20 test
organisms were exposed to the limit concentration and control. Due to
the very poor solubility of the test item (far below 1 mg/L) no test
item analysis was carried out. After 24 and 48 hours of exposure neither
for the treatment (100 mg/L nominal concentration) nor for the control
groups any immobilized daphnids could be observed.
A reference test was carried out with potassium dichromate to determine
the toxicity of the reference item. The EC50-value of the reference item
of 2.07 mg/L after 24 h was within the prescribed concentration range of
2.5 mg/L according to AQS, DIN Guideline 38412 L 30.
Water quality parameters pH-value and dissolved oxygen concentration,
measured at 0 and 48 h, were determined to be within the acceptable
limits. The validity criteria of the test guideline were fulfilled.
The study used as source investigated C.I. Pigment Yellow 1. The study results of the source compound were considered applicable to the target compound and were used for classification and labelling acc. to REGULATION (EC) No 1272/2008. Justification and applicability of the read-across approach (structural analogue) is outlined in the read-across report in section 13 or find a link in cross reference “assessment report”.
With the saturated solution of the submission substance prepared with a
nominal loading of 100 mg/L, no effects were observed in an acute
immobilization test on Daphnia magna according to OECD 202.
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