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EC number: 236-852-7
CAS number: 13515-40-7
Observations in the Test Vessels
Test Duration [h]
*) (1) = Normal behaviour
Cumulative Mortality [%] in the
Test Duration [h]
The acute toxicity of the test item to
fish (zebrafish) was determined according to OECD-Guideline for Testing
of Chemicals No. 203 (1992) and compliant to GLP.
A semi-static limit test with daily
renewal of the test media was conducted with a saturated solution of
1.00 mg/L of the test item. Duration of the test was 96 h. 7 test
organisms were exposed to the limit concentration and the control. Water
quality parameters pH-value, temperature and oxygen-saturation measured
after 0, 24, 48, 72 and 96 h were determined to be within the acceptable
LC-Values with 95 % Confidence
Test duration [h]
p = 95 %
> 1.00 mg/L
Lowest tested concentration level
with 100 % mortality after 96 h
Highest tested concentration level
with 0 % mortality after 96 h
The study used as source investigated C.I. Pigment Yellow 1. The study results of the source compound were considered applicable to the target compound and were used for classification and labelling acc. to REGULATION (EC) No 1272/2008. Justification and applicability of the read-across approach (structural analogue) is outlined in the read-across report in section 13 or find a link in cross reference “assessment report”.
In this study the test item did not cause any effects to zebrafish after
96 h when tested as a saturated solution with a nominal concentration
level of 1.00 mg/L. The LC0 (0 – 96 h) was 1.00 mg/L. No sub-lethal
effects were observed. Due to the very poor solubility of the test item,
qantitative analytical determination by HPLC-DAD was precluded (below
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