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Ecotoxicological information

Toxicity to terrestrial plants

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Link to relevant study record(s)

Description of key information

The long-term toxicity to terrestrial plants was examined in a study in accordance with OECD TG 208, and in compliance with GLP criteria. In this seedling emergence and seedling growth test, five plant species (Brassica napus, Glycine max, Solanum lycopersum, Cucumis sativus, and Avena sativa) were exposed to nominal test concentrations of 0 (control), 12.3, 37.0, 111, 333, and 1000 mg/kg soil dw for 14 days after 50% emergence in the control. A sixth species (Allium cepa) was exposed to nominal test concentrations of 0 (control), 1.37, 4.12, 12.3, 37.0, and 111 mg/kg soil dw for 21 days after 50% emergence in the control. The test item was dissolved in acetone and mixed with fine quartz sand. After evaporation of the acetone, the quartz sand was mixed into the soil. No analytical verification of nominal test concentrations was performed (or required). Measured endpoints consisted of fresh weight (above ground parts of survived plants), emergence, and mortality. In addition, visual phytotoxicity (e.g. chlorosis, necrosis, abnormal growth), and BBCH growth stages were reported. The validity criteria of the test were met.

Most sensitive species in terms of fresh weight was Glycine max with statistically significant reductions (13.5 - 77.6%) at all tested concentrations. The 14-d EC10 was 6 mg/kg soil dw. Most sensitive species in terms of emergence was Solanum lycopersicum which showed an emergence rate of 47% at 1000 mg /kg dry soil. This effect was statistically significant. The emergence rate of all other tested species was not affected. An effect on mortality was observed for Allium cepa at 111 mg/kg dry soil (21%), and for Cucumis sativus and Brassica napus at 1000 mg /kg dry soil (37% and 25%, respectively). The other species showed no statistically significant mortality. Phytotoxic effects observed were chlorosis, necrosis, growth reduction, leaf and stem deformations. Based on these findings, the 14-d EC10 of 6 mg/kg soil dw as observed in Glycine max is used in the assessment.

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for terrestrial plants:
6 mg/kg soil dw

Additional information