Disodium sebacate

Sediment toxicity

Link to relevant study record(s)

Reference

Endpoint:

sediment toxicity: short-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: OSPAR Protocols on Methods for the Testing of Chemicals Used in the Offshore Oil Industry. OSPAR Commission 2005. PartA: A Sediment Bioassay Using an Amphipod Corophiumsp.

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

yes

Remarks:

Acetone (~ 5 mL)

Details on sediment and application:

PREPARATION OF SPIKED SEDIMENT
- Pooling or mixing of different substrates: Natural sediment with organic content of 2.1 % and silt fraction of 68 %.
- Details of spiking: The appropriate amount of test substance was dissolved in ~ 5 mL of acetone. This solution was added to ~ 90 g of predried sediment. After the acetone had dried off in a fume cupboard, the sample was added to ~ 500 g of damp sediment in a plastic bucket.
- Equilibration time: ~ 24 h
- Equilibration conditions: 130 - 150 mL of seawater was added to each buckets and the buckets were shaken (100 rpm).
- Chemical name of vehicle (organic solvent): Acetone
- Evaporation of vehicle before use: Yes

OTHER
- The sediment was homogenized before preparing the test sediment.
- Each test concentration was prepared separately.
- The sediment was split into three beakers, 400 mL of seawater was added to each.
- The beakers were aerated over night in a climate controlled room with 15 °C.

Test organisms (species):

Corophium volutator

Details on test organisms:

TEST ORGANISM
- Source: Biotix (Dag Altin), Trondheim, Norway
- Details on collection: collected 21.10.2009
- Handling of egg masses and larvae: The organisms were transported by car and airplane to Eurofins on 26.10.09.
- Size (range): amphipods were 5 - 10 mm long.

ACCLIMATION
- Acclimation conditions: Temperature 15.0 - 16.4 °C.

Study type:

laboratory study

Test type:

static

Water media type:

saltwater

Type of sediment:

natural sediment

Limit test:

no

Duration:

10 d

Exposure phase:

total exposure duration

Test temperature:

14.4 - 15.8 °C

pH:

initial 8.0 - 8.1

Dissolved oxygen:

Initial: 92-97 % saturation
After 5 d: 91-98 % saturation
After 10 d: 90-96 % saturation

Salinity:

34.4 per mill

Nominal and measured concentrations:

- Test concentrations nominal: 58, 99, 181, and 340 mg/kg sediment (dry weight)

Details on test conditions:

TEST SYSTEM
- Test container: 600 mL glass container
- Sediment volume: ~200 g
- Overlying water volume: ~400 mL seawater
- Aeration: yes

EXPOSURE REGIME
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 3
- No. of replicates per control / vehicle control: 3/3

OVERLYING WATER CHARACTERISTCS
- Type of water: Natural seawater
- Source of water: tap
- Location: Byfjord
- Description of sampling site: unpolluted site at ~ 80 m depths

SOURCE OF NATURAL SEDIMENT
- Location and description of sampling site: Natural sediment from the same unpolluted site as the amphipod was collected and used for the test.

EFFECT PARAMETERS MEASURED
- Measurement of salinity, oxygen, temperature and pH were done at start, after five and after ten days.
- Mortality: After sieving with a mesh size of 0.5 mm the lethality was registered by visual observation.

VEHICLE CONTROL PERFORMED: yes

TEST CONCENTRATIONS
- Test concentrations: 5.6, 10.3, 18.0 and 32.2 mg/kg sediment (dry weight)

Reference substance (positive control):

yes

Remarks:

Fluoranthene

Key result

Duration:

10 d

Dose descriptor:

LC50

Effect conc.:

161 mg/kg sediment dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Key result

Duration:

10 d

Dose descriptor:

LC10

Effect conc.:

39 mg/kg sediment dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

Dicarboxylic acid:
LC10 10d: 39 mg/kg sediment dw
LC90 10d: >340 mg/kg sediment dw

Results with reference substance (positive control):

Floranthene:
LC10 10d: 7.3 mg/kg sediment dw
LC50 10d: 12 mg/kg sediment dw
LC90 10d 19.7 mg/kg sediment dw
NOEC 10d 5.6 mg/kg sediment dw

Description of key information

In a study report on sediment toxicity in marine water on Corophium volutator according to the Guideline of the OSPAR Commission (2005), toxic effects were observed (BASF SE, 2009).

Additional information

In a study report on sediment toxicity in marine water on Corophium volutator according to the Guideline of the OSPAR Commission (2005), toxic effects were observed (BASF SE, 2009).

In a 10 d toxicity study, Corophium volutator in natural seawater sediment were exposed to sebacic acid, according to the OSPAR Protocols on Methods for the Testing of Chemicals Used in the Offshore Oil Industry (OSPAR Commission 2005) (BASF SE, 2009). Corophium volutator were exposed to nominal concentrations of 0 (control), 58, 99, 181, and 340 mg/kg sediment dw under static conditions. The 10 d LC50, based on the observed mortality, was 161.0 mg/kg sediment dw.

However, according to ECHA guidance R7b effects can be extrapolated from the anion or cation, whichever has the most toxic effect, if no data are available on a salt.

If the effect is related to only one of the ions, the classification of the salt should use the effect concentration multiplied by the salt/ion molecular weight ratio.

The molecular weight ratio between salt and ion is 246 : 202 (1.22)

Based on this correction the LC 10 and LC50 for disodium sebacate in the test with the marine species Corophium volutator.was determined to be 47.6 and 196.4 mg/L, respectively.