Registration Dossier
Registration Dossier
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EC number: 235-185-9 | CAS number: 12125-01-8
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
One key study was selected for ammonium toxicity showing a toxicity to Daphnia magna of 101 mg/L (95% CI; 92.4 - 110 mg/l) as NH4Cl.
A number of non-guideline studies performed with sodium fluoride are available. EC50 values for marine invertebrates range from 10.5 - 39 mg/L. EC50 values for daphnids range from 97 - 352 mg/L. Benthic insect larvae were found to be more sensitive, with EC50 values of 26 - 48 mg/L (test with soft water).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 26 mg/L
Marine water invertebrates
Marine water invertebrates
- Effect concentration:
- 10.5 mg/L
Additional information
As ammonium fluoride decomposes in an aqueous environment into ammonium and fluoride ions, toxicity of these two ions has been studied and the most conservative one has been kept for assessment.
Ammonium toxicity was studied in an acute toxicity test, in which Daphnia magna were exposed to the test substance (ammonium chloride) under static conditions. The methodology followed was ASTM E729-8 (similar to OECD 202 guideline).
The result obtained is a 48h EC50 (survival) = 2.94 mg/L (95% CL; 2.70-3.22 mg/L) as NH3-N and calculated as 101 mg/L (95% CI; 92.4-110 mg/l) as NH4Cl.
Fluoride toxicity was studied in various non-guideline studies. Camargo & Tarazona (1991) report that benthic larvae are sensitive to the concentration of fluoride, with EC50 values ranging from 26 - 48 mg/L (actual concentration); however the study was performed in soft water. The EU RAR reviews and summarises the available data on short-term toxicity to aquatic invertebrates. The reported EC50 values for Daphnia sp. range from 97 - 352 mg/L and are based on nominal concentrations. EC50 values of 10.5 - 39 mg/L are reported for marine invertebrates. The ICD summarises the available data on the short-term toxicity of sodium fluoride to aquatic invertebrates and reports EC50 values of 109 - 340 mg/L for freshwater and EC50 values of 30 - 500 mg/L for marine invertebrates.
In conclusion, considering the respective toxicity to aquatic invertebrates in freshwater and marine water for both ammonium and fluoride, the most conservative values are retained as key data for hazard assessment (i.e. fluoride).
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