6-deoxy-L-mannose

Endpoint summary

Description of key information

Skin:

QSAR. Unlikely to be irritating to skin. Reliability = 2

OECD 402, rat. No irritation. Reliability = 1

Human Repeat Insult Patch Test. Irritation in 1 of 106 subjects. Reliability = 2.

Eye: QSAR. Unlikely to be irritating to the eye. Reliability = 2

Key value for chemical safety assessment

Skin irritation / corrosion

Link to relevant study records

Reference

Reference 1

Endpoint:

skin irritation: in vivo

Type of information:

(Q)SAR

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification

Qualifier:

no guideline available

Principles of method if other than guideline:

Times v.2.27.17.6 in QSAR Toolbox
Toolbox prediction report is attached in IUCLID

GLP compliance:

no

Specific details on test material used for the study:

SMILES: CC{P+}(O)C{P+}(O)C{P-}(O)C{P-}(O)C=O

Irritation parameter:

other: QSAR skin irritation/corrosion

Remarks on result:

no indication of irritation

Remarks:

Model indicates that the test substance is unlikely to be irritating to the skin

Interpretation of results:

GHS criteria not met

Conclusions:

Model indicates that the test substance is unlikely to be irritating to the skin

Executive summary:

The Times model for skin irritation/corrosion was used within the QSAR Toolbox. The model indicates that the test substance is unlikely to be irritating to the skin. Additional supporting documentation is provided in the prediction report attached in IUCLID.

Reference 2

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: EPA OPPTS 870.1200 (Acute Dermal Toxicity)

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: EU Method B.3 (Acute Toxicity (Dermal))

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: MAFF Japan Test Guidelines for Agricultural Chemicals 12-Nousan-8147 (2000)

Deviations:

no

GLP compliance:

yes

Species:

rat

Strain:

other: Crl:CD(SD)

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Charles River Laboratories International, Inc., Raleigh, North Carolina, U.S.A.
- Age at study initiation: approximately 9 weeks old
- Weight at study initiation: 299.0-327.9 g (males); 213.2-226-8 g (females)
- Fasting period before study: No
- Housing: Individually in solid-bottom caging with bedding and appropriate species-specific enrichment.
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 6 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C) :20-26ºC (68-79ºF)
- Humidity (%): 30-70%
- Air changes (per hr): Not reported
- Photoperiod (hrs dark / hrs light): approximate 12-hour light/dark cycle

Type of coverage:

semiocclusive

Preparation of test site:

shaved

Vehicle:

water

Controls:

no

Amount / concentration applied:

5000 mg/kg body weight

Duration of treatment / exposure:

24 hours

Observation period:

14 days

Number of animals:

5

Details on study design:

TEST SITE
- Area of exposure: approximately 37 square centimeters
- % coverage: approximately 10 percent of the total body surface area of rats in the 200 - 300 g body weig
ht range
- Type of wrap if used: 2-ply gauze patch. The rats were then wrapped with stretch gauze bandage and
self-adhesive bandage.
REMOVAL OF TEST SUBSTANCE
- Washing (if done): Washed with paper towels soaked in warm water
- Time after start of exposure: Approximately 24 hours
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 5000 mg/kg of body weight
- For solids, paste formed: yes
Details on study design
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: The rats were weighed on test days 1, 8, and 15, and were
observed daily for clinical signs of toxicity and dermal irritation (weekends and holidays excluded for
dermal irritation). The rats were reshaved as needed during the study.
- Necropsy of survivors performed: yes

Irritation parameter:

erythema score

Basis:

other: all animals

Time point:

other: 24-hours to 14 days

Remarks on result:

no indication of irritation

Irritation parameter:

edema score

Basis:

other: all animals

Time point:

other: 24 hours to 14 days

Remarks on result:

other: no oedema observed

Irritant / corrosive response data:

There were no instances of edema or erythema observed.

Other effects:

Refer to acute dermal toxicity study DI.K1.Derm.R.D-21372-673.KD in IULICD section 7.2.3 (Acute toxicity: dermal) for additonal information.

Interpretation of results:

GHS criteria not met

Conclusions:

No instances of erythema or oedema were observed.

Executive summary:

A single dose of the test substance was applied to the shaved, intact skin of 5 male and 5 female rats at a dose level of 5000 mg/kg of body weight. The application site was covered with a semi-occlusive dressing for 24 hours, after which the test substance was removed. The rats were observed for 14 days following application. The rats were necropsied to detect grossly observable evidence of organ or tissue damage at the end of the 15-day test period. There were no incidents of mortality and no overall (test day 1-15) body weight losses among any animals. One male exhibited scabbing on the back (inside of the test site) between test days 6-15 and discoloured skin on the back between test days 6-7. Between test days 12-15, a wound on the right shoulder was observed in one male. Epidermal scaling (inside the test site) was noted in one female between test days 6-8. No other clinical abnormalities were observed. There were no instances of oedema or erythema observed. Gross findings were present in 2/5 male animals, which correspond to the clinical observations noted above. One male had a skin ulcer/erosion present on the back at the outer edge of the treated skin area. The other male had a single skin ulcer/erosion present on the right shoulder. No other gross findings were observed. Under the conditions of this study, the dermal LD50 for the test substance was greater than 5000 mg/kg of body weight when applied to the skin of male and female rats for 24 hours.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Eye irritation

Link to relevant study records

Reference

Endpoint:

eye irritation: in vivo

Type of information:

(Q)SAR

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model, but not (completely) falling into its applicability domain, with adequate and reliable documentation / justification

Qualifier:

no guideline available

Principles of method if other than guideline:

Times v.2.27.17.6 in QSAR Toolbox
Toolbox prediction report is attached in IUCLID

GLP compliance:

no

Irritation parameter:

other: QSAR eye irritation model

Remarks on result:

no indication of irritation

Remarks:

Model indicates that the test substance is unlikely to be irritating to the eye

Interpretation of results:

GHS criteria not met

Conclusions:

Model indicates that the test substance is unlikely to be irritating to the eye.

Executive summary:

The Times model for in vivo eye irritation was used within the QSAR Toolbox. The model indicates that the test substance is unlikely to be irritating to the eye. Additional supporting documentation is provided in the prediction report attached in IUCLID.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Respiratory irritation

Endpoint conclusion

Endpoint conclusion:

no study available

Additional information

The Times model for skin irritation/corrosion was used within the QSAR Toolbox. The model indicates that the test substance is unlikely to be irritating to the skin. In an in vivo, dermal toxicity study, a single dose of the test substance was applied to the shaved, intact skin of 5 male and 5 female rats at a dose level of 5000 mg/kg of body weight. The application site was covered with a semi-occlusive dressing for 24 hours, after which the test substance was removed. The rats were observed for 14 days following application. There were no instances of oedema or erythema observed during the study. Additionally, a leave-on facial product containing 10% of the test substance was examined in a Human Repeated Insult Patch Test (HRIPT) with 48-72 h occlusive patches. Irritation reaction consisting of severe to mild erythema, bulla, colouration, fissuring, and scabbing was observed in only 1 of 106 subjects. Therefore, based on the data available, the test substance is not expected to be irritating to the skin.

 

The Times model for in vivo eye irritation was used within the QSAR Toolbox. The model indicates that the test substance is unlikely to be irritating to the eye.

Justification for classification or non-classification

Based on QSAR and in vivo tests in rats and humans, the test substance is unlikely to be irritating to the skin. Based on QSAR model, the test substance is unlikely to be irritating to the eye.  Therefore, the substance does not need to be classified for skin or eye irritation according to EU Classification, Labelling and Packaging of Substances and Mixtures (CLP) Regulation (EC) No. 1272/2008.