Benzoic acid, 3,3'-[(2,5-dimethyl-1,4-phenylene)bis[imino(1-acetyl-2-oxo-2,1-ethanediyl)azo]]bis[4-chloro-

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

no

Remarks:

Compliance statement mentioned in table of contents but missing

Test material

Test material information

Constituent 1

Reference substance name:

3-[(2E)-2-[1-({4-[(2E)-2-[2-(5-carboxy-2-chlorophenyl)hydrazin-1-ylidene]-3-oxobutanamido]-2,5-dimethylphenyl}carbamoyl)-2-oxopropylidene]hydrazin-1-yl]-4-chlorobenzoic acid

EC Number:

616-083-7

Cas Number:

74351-78-3

Molecular formula:

C30 H26 Cl2 N6 O8

IUPAC Name:

3-[(2E)-2-[1-({4-[(2E)-2-[2-(5-carboxy-2-chlorophenyl)hydrazin-1-ylidene]-3-oxobutanamido]-2,5-dimethylphenyl}carbamoyl)-2-oxopropylidene]hydrazin-1-yl]-4-chlorobenzoic acid

Details on test material:

- Name of test material (as cited in study report): TK 10114/ZP
- Physical state: insoluble brown powder
- Analytical purity: commercial grade
- Lot/batch No.: OP 31
- Expiration date of the lot/batch: 1992-03
- Storage condition of test material: at room temperature

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Bacteria collected from activated sludge of sewage treatment plant of Reinach, Swizterland on the 18th of August 1988
- Preparation of inoculum for exposure: Preparation carried out according to guideline

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

10.7 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

20.8 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Composition of medium: Standard medium according to guideline
- Solubilising agent (type and concentration if used): Nonylphenol 10E05P0 ( 31.2 mg in 100 mL bidist. water)
- Test temperature: 22 ± 2 °C
- Aeration of dilution water: 1200 mL of mineral solution with inoculum were aerated for 24 hours

TEST SYSTEM
- Culturing apparatus: 2 Liter flasks equipped with gas inlet and magnetic stirrer
- Number of culture flasks/concentration: 1
- Measuring equipment: carbon analyser
- Details of trap for CO2 and volatile organics if used: CO2 traps using 200 mL of 0.05 N NaOH

SAMPLING
- Sampling frequency: sampling on days 5, 11, 14, 18, 21, 24, 27 and 28
- Sampling method: determination of trapped CO2

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: no
- Other: blank including vehicle, reference substance

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (CO2 evolution)

Value:

27

Sampling time:

28 d

Remarks on result:

other: test item concentration of 10.7 mg/L

% Degradation 2

Parameter:

% degradation (CO2 evolution)

Value:

3

Sampling time:

28 d

Remarks on result:

other: test item concentration of 20.8 mg/L

BOD5 / COD results

Results with reference substance:

94.4% biodegradation in 28 days

Applicant's summary and conclusion

Interpretation of results:

not inherently biodegradable