[No public or meaningful name is available]

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

17 May 2022 - 18 July 2022

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Appearance Clear and slightly viscous liquid, may solidify at room temperature
Test substance storage Store the containers between 5 and 25º C, in a dry and well-ventilated place, far from sources of heat and direct solar light. Keep far away from ignition points. Keep away from oxidising agents and from highly acidic or alkaline materials.
Stability under storage Stable under normal conditions. Stable under recommended storage conditions

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

5 daphnids per vessel each completely filled with test solution and without headspace
4 replicates with daphnids per treatment group.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20.2°C

pH:

8.15

Details on test conditions:

Test environment Controlled environment cabinet (20°C ± 2°C)
Light regime 16h light : 8h dark
Aeration No aeration of the test solutions occurred throughout the test.
Feeding No feeding

Reference substance (positive control):

yes

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The toxic effect of the test substance to the freshwater invertebrate Daphnia magna was investigated in a closed static limit test. Under the experimental conditions and based upon analytically confirmed nominal concentrations, the 48-hour EC50 value was determined to be higher than 100 mg/L.

Results with reference substance (positive control):

The 24h-EC50 was 1.223 mg/L.

Validity criteria fulfilled:

yes

Conclusions:

The toxic effect of the test substance to the freshwater invertebrate Daphnia magna was investigated in a closed static limit test. Under the experimental conditions and based upon analytically confirmed nominal concentrations, the 48-hour EC50 value was determined to be higher than 100 mg/L.

Description of key information

The toxic effect of the test substance to the freshwater invertebrate Daphnia magna was investigated in a closed static limit test. Under the experimental conditions and based upon analytically confirmed nominal concentrations, the 48-hour EC50 value was determined to be higher than 100 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

> 100 mg/L