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EC number: 203-584-7 | CAS number: 108-45-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to terrestrial plants
Administrative data
- Endpoint:
- toxicity to terrestrial plants
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Induction of Somatic Mutations in Tradescantia clone 4430 by Three Phenylenediamine Isomers and the Antimutagenic Mechanisms of Diethyldithiocarbamate and Ammonium Vanadate
- Author:
- Gichner T, Cabrera Lopez G, Wagner ED, and Plewa MJ.
- Year:
- 1 994
- Bibliographic source:
- Mutation Research; 306: 165-172
Materials and methods
- Principles of method if other than guideline:
- - 50 cuttings of Tradescantia clone 4430 10 -15 cm long with inflorescences per treatment group.
- From each inflorescence, 4 -6 flower buds were removed, and 20 ul of test solution was applied in the resulting cup.
- Negative controls were citrate phosphate buffer pH 5.
- For each concentration, 10 flowers were scored daily. One or more contiguous pink cells were considered to have resulted from one mutation event.
- Frequency of mutations was expressed as the average percentage of pink mutations scored on days 9 -14 after treatment. - GLP compliance:
- no
Test material
- Reference substance name:
- m-phenylenediamine
- EC Number:
- 203-584-7
- EC Name:
- m-phenylenediamine
- Cas Number:
- 108-45-2
- Molecular formula:
- C6H8N2
- IUPAC Name:
- m-phenylenediamine
- Details on test material:
- - Name of test material (as cited in study report): m-phenylenediamine
- Physical state: Solid
- Purchased from Sigma Chemical Co. (St. Louis, MO, USA)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- Vehicle: 0.2 M citrate-phosphate buffer (pH = 5)
Test organisms
- Species:
- other: Tradescantia clone 4430
- Details on test organisms:
- Hybrid of T. subacaulis X T. hirsutiflora cultivated at the Institute of Experimental Botany, Prague
Study design
- Test type:
- other: Mutagenicity assay
- Study type:
- laboratory study
- Total exposure duration:
- 15 d
- Post exposure observation period:
- Mutations scored on days 9 - 14 post-exposure
Test conditions
- Test temperature:
- 18-22° C
- Details on test conditions:
- Test system:
- Amount of soil: None
- No. of plants: 50 cuttings, 10-15 cm long, per treatment group
- No. of replicates per treatment group: 10 flowers scored daily for each concentration and control
- No. of replicates per vehicle control: Not specified
Growth conditions:
- Photoperiod: 18 h
- Day/night temperatures: 18-22° C
- Water source/type: Cuttings cultivated in beakers with tap water for 15 days
Vehicle control performed: yes - Nominal and measured concentrations:
- 25, 50, 100, 150, 200, 300 and 400 mM m-phenylenediamine dissolved in 0.2 M citrate-phosphate buffer, plus control (citrate-phosphate buffer only).
Results and discussion
Effect concentrationsopen allclose all
- Species:
- other: Tradescantia clone 4430
- Dose descriptor:
- other: mutagenicity
- Effect conc.:
- other: positive
- Basis for effect:
- other: pink stamen hair mutations
- Species:
- other: Tradescantia clone 4430
- Dose descriptor:
- LOEC
- Effect conc.:
- > 300 other: mM
- Basis for effect:
- other: flowering repression
- Details on results:
- A concentration dependent increase in pink stamen hair mutations was seen in the concentration range of 25-300 mM m-phenylenediamine.
m-phenylenediamine was found to be toxic at concentrations above 300 mM, and repressed flowering.
At 300 mM, m-phenylenediamine induced approximately a 10-fold increase in the mutant frequency over the spontaneous control. - Reported statistics and error estimates:
- The statistical approach outlined by Underbrink et al. 1973 was used to analyze the data from the stamen hair assays.
ANOVA test was used to test for differences among treatment groups, and if a significant F value was obtained, each treatment group and its
corresponding negative control was tested for significance using Dunnett's test for multiple comparisons.
The mutagenic potency of m-phenylenediamine was 1.43 stamen hair mutants/umole.
Applicant's summary and conclusion
- Conclusions:
- Under the test conditions employed in this study, m-phenylenediamine was mutagenic in Tradescantia clone 4430 stame hair cells when compared to the control. The mutagenic potency of m-phenylenediamine was 1.43 stamen hair mutants/umole.
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