Chromium trioleate

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Chromium trioleate

EC Number:

244-256-3

EC Name:

Chromium trioleate

Cas Number:

21178-63-2

Molecular formula:

C18 H34 O2 .1/3 Cr

IUPAC Name:

chromium(3+) tris((9Z)-octadec-9-enoate)

Details on test material:

Chrom (III)-oleat
Oleic acid Cr III-salt
Batch No.: 5
Product No.: 78623
Purity: no data

Report of the Analytical Laboratory No.: 99L00034

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

The activated sludge inoculum used in this study originated from laboratory wastewater treatment plants fed with municipal sewage. The inoculum was washed, brought to a concentration of 5g/L dry substance and aerated for 24 hours. 50 mL were added to a total volume of 250 mL to obtain a concentration of 1g/L dry substance in the test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

30 min

Test conditions

Test temperature:

20 +/- 2°C

pH:

before correction: 6.2
after correction: 7.8
after 30 minutes incubation: 7.6

Dissolved oxygen:

Oxygen concentration immediately before measurement: 7.6 mg/L

Nominal and measured concentrations:

The compound was tested at the concentration of 1000 mg/L (nominal).

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer-vessel
- Test volume: 250 mL
- Synthetic medium: 8 mL/vessel 100-fold concentration OECD medium
- Duration of the measurement of oxygen consumption: 6-10 min

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

30 min

Dose descriptor:

EC0

Effect conc.:

>= 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Results with reference substance (positive control):

EC20 (30min) ca. 2 mg/L
EC50 (30min) ca. 8 mg/L
EC80 (30min) ca. 70 mg/L

Any other information on results incl. tables

No significant inhibition of respiration was measured at the tested concentration of 1000 mg/L (nominal).