4-amino-6-chlorotoluene-3-sulphonic acid

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP guideline study without analytical test item concentration verification

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Reference 2

Reference Type:

other company data

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

other: National Industrial Standard Test Guideline DIN 38412, part 9

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-amino-4-chloro-5-methyl-benzensulfonic acid sodium salt

IUPAC Name:

2-amino-4-chloro-5-methyl-benzensulfonic acid sodium salt

Details on test material:

- Name of test material (as cited in study report): Chlor-p-Toluidinsaeure Natriumsalz
- Analytical purity: no data

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:stock solution with a concentration of 1000 mg/L
- Differential loading: no
- Controls: OECD medium
- Evidence of undissolved material (e.g. precipitate, surface film, etc): none reported

Test organisms

Test organisms (species):

Ankistrodesmus bibraianus

Details on test organisms:

TEST ORGANISM
- Common name: Green alga
- Strain: B 61.81
- Source (laboratory, culture collection): in-house culture
- Age of inoculum (at test initiation): 72 h
- Method of cultivation: cultures in 250 mL Erlenmeyer flasks, fill volume 100 mL, OECD medium, temperature of 20 °C

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

20 °C

pH:

8.46 - 9.2

Nominal and measured concentrations:

nominal: control, 1.9, 3.9, 7.8, 15.6, 31.3, 62.5, 125, 250, 500 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: reaction vessels with flat bottom
- Material, size, headspace, fill volume: 10 mL fill volume
- Initial cells density: 10,000 cells/mL
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Photoperiod: continuous
- Light intensity and quality: 120 µE/(m2*s)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : fluorescence of chlorophyll a at 685 nm as measure for biomass
- Determination of cell concentrations: fluorimeter
- Chlorophyll measurement: fluorescence

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: fluorescence of chlorophyll a at 685 nm

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: fluorescence of chlorophyll a at 685 nm

Details on results:

- Other: fluorescence in the blanks: 1 - 2