Dimethyl 3-oxoglutarate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2002

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Dimethyl 3-oxoglutarate

EC Number:

217-385-8

EC Name:

Dimethyl 3-oxoglutarate

Cas Number:

1830-54-2

Molecular formula:

C7H10O5

IUPAC Name:

dimethyl 3-oxoglutarate

Details on test material:

- Name of test material (as cited in study report): LZ 409
- Molecular formula (if other than submission substance):
- Molecular weight (if other than submission substance):
- Smiles notation (if other than submission substance):
- InChl (if other than submission substance):
- Structural formula attached as image file (if other than submission substance): see Fig.
- Substance type: organic
- Physical state: light yellow liquid
- Analytical purity:
- Impurities (identity and concentrations):
- Composition of test material, percentage of components:
- Isomers composition:
- Purity test date:
- Lot/batch No.:
- Expiration date of the lot/batch:
- Radiochemical purity (if radiolabelling):
- Specific activity (if radiolabelling):
- Locations of the label (if radiolabelling):
- Expiration date of radiochemical substance (if radiolabelling):
- Stability under test conditions:
- Storage condition of test material: At room temperature in the dark
- Other:

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Serial dilution of an initial stock solution with a nominal concentration of 100 mg/l, the highest test concentration.
- Eluate:
- Differential loading:
- Controls:
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): water
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)):
1500 mg of LZ409 to 15 litres of water and 1.5 litres was diluted to 15 litres, test concentration of 10 mg/l, 1.0 and 0.1 mg/l.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): none

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Strain:
- Source: Donnington Trout Farm
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): 4-6 cm
- Weight at study initiation (mean and range, SD):
- Method of breeding:
- Feeding during test
- Food type:
- Amount:
- Frequency:

ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions (same as test or not): same
- Type and amount of food: standard fish diet
- Feeding frequency:
- Health during acclimation (any mortality observed): good health and free from any apparent malformation

QUARANTINE (wild caught)
- Duration:
- Health/mortality:

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Remarks on exposure duration:

every 24 h

Post exposure observation period:

1, 4, 24, 48, 96 h

Test conditions

Hardness:

64.0-69.2 mg CaCO3I-1

Test temperature:

15.3-16.3 Degree Celsius

pH:

6.77-7.62

Dissolved oxygen:

78.4-93.9 %

Salinity:

Conductivity: 155.2-184.8 microS

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume:
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel: 7 fish
- No. of vessels per concentration (replicates):
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates):
- Biomass loading rate:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks:

1/7 fish

Effect concentrations 2

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks:

1/7 fish

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks:

2/7 fish

Effect concentrations 4

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

ca. 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: no mortality

Details on results:

Two fish were observed to be moribound in the 100 mg/L test tank. These were removed to avoid unnecessary distress. The firts was removed at 1610 h on the second day of the test (cs. 28 h after introduction). The second was removed at 0935 h on the last day of the test (ca. 93.5 h after initiation). No mortality was observed in any other test or control tanks.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 96 h LC50 of the substance to rainbow trout, under semi-static conditions, was estimated to be > 100 mg/L the highest concentration tested.
The NOEC was estimated to be 10 mg/L, as this was the highest contentration where no mortality or test item related abnormalities were observed after 96 h exposure.