N,N-diethylaniline

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result were performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

Study was conducted to access the effect of test chemical to zebra fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

The test solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous stirring for 24 hours. From this stock solution, further test concentration was prepared for achieving test concentrations of 25 mg/L, 32.5 mg/L, 42.25 mg/L, 54.92 mg/L & 71.4 mg/L. respectively.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD): average 2.7 cm
- Weight at study initiation (mean and range, SD): average 0.272 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 12 hours light and 12-hour darkness
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

50.75 mg of CaCO3

Test temperature:

24°C

pH:

6.91

Dissolved oxygen:

7.2 mg/L

Nominal and measured concentrations:

Nominal concentration: 25 mg/L, 32.5 mg/L, 42.25 mg/L, 54.92 mg/L & 71.4 mg/L.

Details on test conditions:

TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 12 hours light and 12-hour darkness

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

42.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other:

Remarks:

Not known

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other:

Remarks:

Not known

Sublethal observations / clinical signs:

Mortality and symptoms

Nominal Concentration (mg/l)	Number of fish	Mortality (cumulated) after
		24h	48h	72h	96h
0 (control)	8	-	-	-	-
25	8	-	-	-	-
32.5	8	-	-	1	1
42.25	8	2	1	1	-
54.92	8	3	5	-	-
71.4	8	2	6	-	-

 

Visible Symptoms: Fishes were moving slowly as compared to the control.

 

Nominal Concentration (mg/l)	Number of fish	Symptoms after
		24h	48h	72h	96h
0 (control)	8	NS8	NS8	NS8	NS8
25	8	M8	M8	M8	M8
32.5	8	M8	M8	M7	M6
42.25	8	M6	M5	M4	M4
54.92	8	M5	-	-	-
71.4	8	M6	-	-	-

 

 

Explanation of symptoms:

A: apathy

N: narcotic-like state

C: swimming near water surface

D: swimming at the bottom of bowl

M: Slow movements compared to control

NS: No abnormal symptoms

Number behind symbol for symptom= number of affected fish

 pH and oxygen content:

Nominal Concentration (mg/l)	pH readings after
	24h	48h	72h	96h
0 (control)	7.4	7.2	7.2	6.5
25	7.0	6.8	6.6	6.5
32.5	6.9	6.5	6.3	6.1
42.25	6.6	6.4	6.2	6.1
54.92	6.7	-	-	-
71.4	6.5	-	-	-

 

Nominal Concentration (mg/l)	Oxygen content (mg/l) after
	24h	48h	72h	96h
0 (control)	6.5	6.5	6.2	6.1
25	6.5	6.5	6.1	6.1
32.5	6.5	6.5	6.1	6.1
42.25	6.4	6.4	6.1	6.0
54.92	6.4	-	-	-
71.4	6.5	-	-	-

 

Test temperature

Nominal Concentration (mg/l)	Temperature (°C) after
	24h	48h	72h	96h
0 (control)	23	23	23	23
25	23	23	23	23
32.5	23	23	23	23
42.25	23	23	23	23
54.92	23	-	-	-
71.4	23	-	-	-

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test chemical on Zebra Fish Danio rerio was determine to be 42.25 mg/L.

Executive summary:

Study was conducted to access the effect of test chemical to zebrafishDanio rerio. Test was conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The range of test concentration was taken to evaluate effects i.e.,25 mg/L, 32.5 mg/L, 42.25 mg/L, 54.92 mg/L & 71.4 mg/L. Experiment was performed for the period of 96 hours in a static conditions.

From the results obtained, it is observed that the median lethal concentration i.e., LC50 was to be 42.25 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be classified into aq aquatic chronic 3 as per the CLP classification criteria.

Description of key information

Study was conducted to access the effect of test chemical to zebrafishDanio rerio. Test was conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The range of test concentration was taken to evaluate effects i.e.,25 mg/L, 32.5 mg/L, 42.25 mg/L, 54.92 mg/L & 71.4 mg/L. Experiment was performed for the period of 96 hours in a static conditions.

From the results obtained, it is observed that the median lethal concentration i.e., LC50 was to be 42.25 mg/l .

Based on the LC50, it can be consider that the chemical was toxic and can be classified into aq aquatic chronic 3 as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

42.25 mg/L

Additional information

Study was conducted to access the effect of test chemical to zebrafishDanio rerio. Test was conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The range of test concentration was taken to evaluate effects i.e.,25 mg/L, 32.5 mg/L, 42.25 mg/L, 54.92 mg/L & 71.4 mg/L. Experiment was performed for the period of 96 hours in a static conditions.From the results obtained, it is observed that the median lethal concentration i.e., LC50 was to be 42.25 mg/l.

 

A study from peer reviewed journal states that the median lethal concentration (LC50) value of test chemical to fish (Oryzias latipes) in a 24 study on mortality effect was determined to be 40mg/L.

Another study from peer reviewed journal states The median lethal concentration (LC50) value of test chemical to fish (Oryzias latipes) in a 48 hr study on mortality effect was determined to be 25 mg/L.

Data from the authoritative database stats that, The test was conducted based on the oecd guidelines 203 for the period of 96 h.The median lethal concentration, LC50 value of test chemical to fish was determined to be 26 mg/L.

Based on the various outcomes the test chemical can be classified into the Aq chronic category 3