Iron, bis(glycinato-.kappa.N,.kappa.O)-

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2019

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2019

Report date:

2019

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

bis(λ⁴-iron(2+)) tetrakis(2-aminoacetate)

EC Number:

606-444-7

Cas Number:

20150-34-9

Molecular formula:

C4H8FeN2O4

IUPAC Name:

bis(λ⁴-iron(2+)) tetrakis(2-aminoacetate)

Test material form:

solid

Test solutions

Vehicle:

yes

Remarks:

Based on the in house solubility/suspensibility test , iron glycinate was soluble in OECD algal medium. Hence, OECD algal media was selceted as the carrier for preparation of test item formu altion.

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

14.81 mg/L

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

48.53 mg/L

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

4 mg/L

Effect concentrations 4

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

7.7 mg/L

Details on results:

Based on the result of range finding study, main study was performed with the test concentration of 4.0, 7.7, 14.6, 27.7, 52.6 and 100 mg/l of iron glycinate along with the control and surrogate groups.
The algal growth (cell density) was assesed at 24, 28 and 72 hours post-exposure. Exponentially g
rowing alga cells (5346 cells/ mL)were eposed to the test concentrations.
The percent growth rate inhibition as compared with the control is found to be 0.54, 0.45, 2.36, 9.47, 31.31 and 100% at the tested concentrations of 0.01, 0.1, 1.0, 10, 50 and 100mg/l respectively.
The percent inhibition is yield as compared with the control is found to be 3.31; 8.52,; 26.05; 85.55; 94.37 and 100% at the tested concentration of 4.0; 7.7; 14.6; 27.7; 52.6; and 100 mg/l respectively.
During the main study exposure, the dose sampes of iron glycinate were analysed for dose f
ormulation for ICP-OES and the result are found to be within the acceptable range of +/_ 20% to the nominal concentration.

Growth rate (ErC10)
The 72h EC10 value for growth rate ErC10 is 14,81 mg/l. The 95% convidence interval value was
found to be lower limit of 12.41 mg/l and upper limit of 17.68 mg/l.

Growth rate (ErC50)
The 72h EC10 value for growth rate ErC50 is 48,53 mg/l. The 95% convidence interval value was
found to be lower limit of 40.78 mg/l and upper limit of 57.74 mg/l.

Yield (EyC10)
The 72h EC10 value for yield EyC10 is 7.11 mg/l (nominal). The 95% convidence interval value was found to be lower limit of 5.23 mg/l and upper limit of 9,67 mg/l.

Yield (EyC50)
The 72h EC50 value for yield EyC50 is 17.43 mg/l (nominal). The 95% convidence interval value was found to be lower limit of 14.36 mg/l and upper limit of 21.15 mg/l.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

EC10=14.81 (growth rate)
EC50=48.53 mg/l (growth rate)
NOEC=4 mg/l
LOEC=7.7 mg/l