Sodium 2-hydroxy-3-[(2-hydroxyethyl)[2-[(1-oxo-9-octadecenyl)amino]ethyl]amino]propanesulphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992-01-06 to 1992-01-10

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not applicable

GLP compliance:

no

Analytical monitoring:

not specified

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Direct dispersion in water
- Controls: 1 replicate test water only)
- Evidence of undissolved material: Not specified

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Source: Parkwood Trout Farm, Kent, U.K.
- Length at study initiation: 5.4 +/- 0.5 cm
- Weight at study initiation: 1.77 +/- 0.33 g
- Medication: None
- Mortalities: Zero in the 7 days prior to the start of the test

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

Not performed

Hardness:

50 mg/L as CaCO3 (dilution water)

Test temperature:

14 °C

pH:

Not specified

Dissolved oxygen:

Not specified

Salinity:

Not applicable

Conductivity:

Not specified

Nominal and measured concentrations:

Nominal: 10 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Not specified
- Aeration: Yes
- Renewal rate of test solution: Daily renewal
- No. of vessels per concentration: 2
- No. of vessels per control: 1
- Animals per test vessel: 10
- Biomass loading rate: 0.89 g bodyweight/litre

TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: Dechlorinated tap water (prepared with Sodium thiosulphate)

EFFECT PARAMETERS MEASURED: Mortality after 24, 48, 72 and 96 hours of exposure

TEST CONCENTRATIONS
- Range finding study : Yes
- Test concentrations in range finding study: 1, 10 and 100 mg/L
- Control: Blank control
- Initial population: 2 animals/vessel
- Results used to determine the conditions for the definitive study: Yes

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Table 1: Results of the main study

Concentration (mg/L)	Cumulative Mortality (initial population = 10)
	24 hours	48 hours	72 hours	96 hours
Control	0	0	0	0
10 R1	0	0	0	0
10 R2	0	0	0	0

R1 - R2: Replicates R1 - R2

Table 2: Results of the range finding study

Concentration (mg/L)	Cumulative Mortality (initial population = 2)
	24 hours	48 hours	72 hours	96 hours
Control	0	0	0	0
1.0	0	0	0	0
10	0	0	0	0
100	2	2	2	2

Validity criteria fulfilled:

not specified

Conclusions:

In an Acute Toxicity Test similar to OECD Guideline 203, the 96-h LC50 of the test item was determined to be > 10 mg/L.

Executive summary:

In a semi-static test similar to OECD Guideline 203, the acute toxicity of the test item to fish (Onchorhynchus mykiss) was assessed at a limit concentration of 10 mg/L (two replicates) over a duration of 96 hours. In addition, a negative control (without the test item, 1 replicate) was performed. As test medium dechlorinated tap water was used. Test solutions were prepared by direct dispersion of the test item in water. As test parameter, mortality was recorded after 24, 48, 72 and 96 hours of exposure. In result, the 96-h LC50 of the test item was determined to be > 10 mg/L.  

Description of key information

In an Acute Toxicity Test similar to OECD Guideline 203, the 96-h LC50 of the test item was determined to be > 10 mg/L.

Additional information

In a semi-static test similar to OECD Guideline 203, the acute toxicity of the test item to fish (Onchorhynchus mykiss) was assessed at a limit concentration of 10 mg/L (two replicates) over a duration of 96 hours. In addition, a negative control (without the test item, 1 replicate) was performed. As test medium dechlorinated tap water was used. Test solutions were prepared by direct dispersion of the test item in water. As test parameter, mortality was recorded after 24, 48, 72 and 96 hours of exposure. In result, the 96-h LC50 of the test item was determined to be > 10 mg/L.