Phenol, 4-isocyanato-, 1,1',1''-phosphorothioate, reaction p roducts with 3-(trimethoxysilyl)-N-(3-(trimethoxysilyl)propy l)-1-propanamine

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2007

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD GLP Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: 92/69/EWG, C.2; OECD 202 (2004)

GLP compliance:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

Identity and concentration of auxiliary solvent for dispersal: none - WAF test

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Details on results:

The 48-Hour EL*50 for the test material to Daphnia magna based on nominal loading
rates was greater than 100 mg/l loading rate WAF and correspondingly the No Observed Effect
Loading rate was 100 mg/l loading rate WAF.
It was considered unnecessary and unrealistic to test at loading rates in excess of 100 mg/l.
Samples of the control and 100 mg/l loading rate WAF were taken at 0 (fresh media) and 48 hours
(old media) for Total Organic Carbon (TOC) analysis. Analysis of the test preparations at 0 hours
showed measured test concentrations to be 10.8 (Replicates R1-R2 pooled) and 10.2 mg/l
(Replicates R3-R4 pooled). After 48 hours, analysis showed measured test concentrations to be
9.7 (Replicates R1-R2 pooled) and 9.4 mg carbon/l (Replicates R3-R4 pooled). No significant
decline in TOC was observed over the duration of the test.
As the test material is a complex mixture, the toxicity cannot be attributed to one or any of the
components in the test material but to the test material as a whole. Therefore the results were
based on nominal loading rates only.

Results with reference substance (positive control):

The 48-Hour EC50 for the reference material to Daphnia magna based on nominal concentrations
was 0.85 mg/l with 95% confidence limits of 0.76 - 0.96 mg/l. The No Observed Effect
Concentration was 0.56 mg/l.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48-Hour EL*50 for the test material to Daphnia magna based on nominal loading rates was greater than 100 mg/l loading rate WAF and correspondingly the No Observed Effect Loading rate was 100 mg/l loading rate WAF.