Reaction mass of m-terphenyl and o-terphenyl

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2017-2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Remarks:

The concentration and stability of the test item in the test preparations was verified by chemical analysis at 0, 24, 72 and 96 hours.

Details on sampling:

- Concentrations: Nominal concentrations of 10, 18, 32, 56 and 100 % v/v.

Vehicle:

no

Test organisms (species):

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

No additional post exposure period implemented in test design.

Salinity:

Not relevant for freshwater study

Nominal and measured concentrations:

The concentration and stability of the test item in the test preparations was verified by chemical analysis at 0, 24, 72 and 96 hours.

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

6.7 µg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% confidence limits: 6.0 to 7.4 µg/L

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

6 µg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LOEC

Effect conc.:

7.4 µg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Mortality of control: No lethal or sub-lethal effects were observed in any control fish.
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: There was a small amount of test item measured in the control sample at the 72 hours (fresh). This is considered to have been post sampling contamination as the 96 hour (old) sample had results of less that the limit of quantification. Therefore considered not to have impacted the outcome of the study. No sub lethal effects were observed in any control fish.
- Effect concentrations exceeding solubility of substance in test medium: Not applicable.

Sublethal observations / clinical signs:

  Table 1: Cumulative mortality for the different concentration levels of the test compound:

Nominal Concentration (% v/v)	Cumulative Mortality (Initial Population = 7)							% Mortality
	1 Hour	3 Hours	6 Hours	24 Hours	48 Hours	72 Hours	96 Hours	96 Hours
Control	0	0	0	0	0	0	0	0
10	0	0	0	0	0	0	0	0
18	0	0	0	0	0	0	0	0
32	0	0	0	0	0	0	0	0
56	0	0	0	0	0	0	0	0
100	0	0	0	0	0	7	7	100

 

  

Table 2: Analytical result:

Concentration (% v/v) Sat Sol	0 hours	24 hours old	72 hours fresh	96 hours old
	Measured Concentration (mg/L)	Measured Concentration (mg/L)	Measured Concentration (mg/l)	Measured Concentration (mg/L)
Control	<LOQ	<LOQ	0.00161	<LOQ
10	0.000972	0.000432	0.00223	0.000931
18	0.00186	0.000897	0.00316	0.00178
32	0.00355	0.00172	0.00614	0.00329
56	0.00628	0.00324	0.0101	0.00552
100	0.0123	0.00442	-	-

LOQ = 0.00030 mg/L

 

Table 3: Effect concentrations of the acute fish toxicity study.

(Hours)	LC50 (µg/L)	95% Confidence Limits (µg/L)			NOEC (µg/L)	LOEC (µg/L)
96	6.7	6.0	-	7.4	6.0	7.4

 

 

 

 

 

 

 

 

Validity criteria fulfilled:

yes

Conclusions:

Acute toxicity of the test compound to freshwater fish was tested according to OECD Guideline 203.
Following results have been obtained after an exposure period of 96 hours: LC50 = 6.7 µg/L (95% confidence limits: 6.0 to 7.4 µg/L), NOEC = 6.0 µg/L, LOEC = 7.4 µg/L.

Executive summary:

Acute toxicity of the test compound to freshwater fish was tested according to OECD Guideline 203 and GLP. Nominal concentrations of 10, 18, 32, 56 and 100 % v/v have been investigated.

The concentration and stability of the test item in the test preparations was verified by chemical analysis at 0, 24, 72 and 96 hours.

At the highest nominal concentration level of 100 % v/v, measured test compound concentration was 12.3 µg/L (0 hours, i.e. at test begin) and 4.42 µg/L (after 24 hours), respectively. LOC was 0.30 µg/L.

Following results have been obtained after an exposure period of 96 hours: LC50 = 6.7 µg/L (95% confidence limits: 6.0 to 7.4 µg/L), NOEC = 6.0 µg/L, LOEC = 7.4 µg/L.

Time Point (Hours)	LC50 (µg/L)	95% Confidence Limits (µg/L)			NOEC (µg/L)	LOEC (µg/L)
96	6.7	6.0	-	7.4	6.0	7.4

 

 

 

 

 

 

 

 

Description of key information

OECD 203, GLP, LC50 (96 h) = 0.0067 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.007 mg/L

Additional information

Acute toxicity of the compound reaction mass of m-terphenyl and o-terphenyl was investigatedaccording to OECD Guideline 203.

Following results have been obtained after an exposure period of 96 hours: LC50 = 6.7 µg/L (95% confidence limits: 6.0 to 7.4 µg/L), NOEC = 6.0 µg/L, LOEC = 7.4 µg/L.