Bis[2-(diethylamino)ethyl] adipate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Bis[2-(diethylamino)ethyl] adipate

EC Number:

240-610-6

EC Name:

Bis[2-(diethylamino)ethyl] adipate

Cas Number:

16545-00-9

Molecular formula:

C18H36N2O4

IUPAC Name:

1,6-bis[2-(diethylamino)ethyl] hexanedioate

impurity 1

Reference substance name:

2-diethylaminoethanol

EC Number:

202-845-2

EC Name:

2-diethylaminoethanol

Cas Number:

100-37-8

Molecular formula:

C6H15NO

IUPAC Name:

2-(diethylamino)ethanol

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

The water-accommodated fraction (WAF) was prepared for the test. This was done by weighing the nominal loads of 100 / 46 / 22 / 10 / 4.6 mg/L on the corresponding amount of dilution water and stirring for 24 hours. The lower phases were used for the test.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Species Daphnia magna
Authority STRAUS
Strain Berlin
Sex female
Age between 0 and 24 hours
Origin Umweltbundesamt Berlin
In-house breeding since 27. September 2007
Selection of the test system was made following the proposal of the guidelines

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Test temperature:

19.7 – 20.4°C

pH:

7.7

Dissolved oxygen:

8.8 mg/L

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 122.5 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

other: WAF

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 122.5 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

other: WAF

Basis for effect:

mobility

Effect concentrations 3

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 122.5 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

other: WAF

Basis for effect:

mobility

Any other information on results incl. tables

¨             Immobilisation in the controls may not exceed 10 %.
Immobilisation in the controls was 0 %.

¨             The concentration of dissolved oxygen at the end of the test must be at least 3 mg/L. The lowest concentration of dissolved oxygen at the end of the test was 8.7 mg/L.

One valid experiment was performed. The study was performed using 5 concentrations ranging from 4.6 to 100 mg/L. For each test concentration, 20 Daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised Daphnia were counted. One Daphnia was immobilised at 10 mg/L, two Daphnia were immobilised at 46 mg/L and three Daphnia were immobilised at 100 mg/L of test item. None of the animals was immobilised in the blank control. Potassium dichromate K₂Cr₂O₇ (CAS No. 7778-50-9) was used as positive controlin a current reference study to assure that the test conditions are reliable. At the start and at the end of the test, the content of the test item in the test solutions was determined using measurement of dissolved organic carbon (DOC). The measured concentration based on DOC measurement was between 116 and 129% of the nominal concentration at the beginning of the test and between 112 and 156% of the nominal concentration at the end of the test. Therefore, the determination of the results was based on the geometric mean of the measured concentrations. No observations were made which might cause doubts concerning the validity of the study outcome. All validity criteria were met. The result of the test is considered valid.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 24h- and 48h-EC50 value was mathematically not determinable. However, the 24h- and 48h-EC50 values can be given in a range (higher than highest tested concentration), since 50% of immobilisation were not reached at the highest tested concentration at the end of the test.
48h-NOEC≥122.5 mg/L
48h-LOEC > 122.5 mg/L
24h-EC50> 122.5 mg/L
48h-EC50> 122.5 mg/L

Executive summary:

One valid experiment was performed. The study was performed using 5 concentrations ranging from 4.6 to 100 mg/L. For each test concentration, 20 Daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised Daphnia were counted. One Daphnia was immobilised at 10 mg/L, two Daphnia were immobilised at 46 mg/L and three Daphnia were immobilised at 100 mg/L of test item. None of the animals was immobilised in the blank control. Potassium dichromate K₂Cr₂O₇(CAS No. 7778-50-9) was used as positive controlin a current reference study to assure that the test conditions are reliable. At the start and at the end of the test, the content of the test item in the test solutions was determined using measurement of dissolved organic carbon (DOC). The measured concentration based on DOC measurement was between 116 and 129 % of the nominal concentration at the beginning of the test and between 112 and 156 % of the nominal concentration at the end of the test. Therefore, the determination of the results was based on the geometric mean of the measured concentrations. The following results were determined for the test itemBis[2-(diethylamino)ethyl]adipate(species:Daphnia magna).

 

48h-NOEC≥122.5 mg/L
48h-LOEC > 122.5 mg/L
24h-EC₅₀> 122.5 mg/L
48h-EC₅₀> 122.5 mg/L