[2-[[2-cyano-3-[4-[ethylbenzylamino]phenyl]-1-oxoallyl]oxy]ethyl]trimethylammonium chloride

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

06 December 2017 to 19 January 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

Analytical samples were analysed from all test item concentrations and control at test start and after 48 hours from aged solutions. samples (10 mL) were stored deep-frozen (≤ - 18 °C) until analysis. In the analytical laboratory, the samples were thawed to ambient temperature, mixed with 10 mL acetonitrile and shaken well using a Vortex-Mixer. The samples were then diluted with acetonitrile/test medium (1:1, v/v) prior to analysis by HPLC-MS/MS.

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Clone 5
- Source: in house originating from Federal Environment Agency in Berlin/Germany.
- Age of parental stock: < 24 h
- Feeding during test: none

CULTURE CONDITIONS
- Conditions: single culture (1 daphnid per 100 mL) in Elendt M4 medium. The pH-value of the aerated water was within a range of 6.0 – 9.0. The dissolved oxygen was above 60 % saturation and the total hardness 140 - 250 mg/L (as CaCO3)
- Type and amount of food: single cell green algae (Desmodesmus subsp.)
- Feeding frequency: at least 3 times per week

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

214 mg/L as CaCO3

Test temperature:

19.2-20.8 °C

pH:

6.64-7.77

Dissolved oxygen:

7.3-9.4 mg/L (excluding O2 concentration in flasks were no survivors were present)

Salinity:

NA

Nominal and measured concentrations:

Nominal: 0, 6.25, 12.5, 25.0, 50.0 and 100 mg/L
Measured (geometric mean): ND, 4.75, 9.75, 20.3, 42.5 and 86.0 mg/L; in fresh solutions 87-91% of nominal, in old solutions 66-82% of nominal

Details on test conditions:

TEST SYSTEM
- Test vessel: 50 mL all glass with screw cap
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ELENDT M4 medium according to the guidance

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 light/ 8 dark
- Light intensity: no data

EFFECT PARAMETERS MEASURED : immobility at 0, 24 and 48 hours

RANGE-FINDING STUDY
- Test concentrations: Semi-static at 0, 1, 10 and 100 mg/L in a closed system
- Results used to determine the conditions for the definitive study: immobility after 24 and 48 h: 0. 0. 0 and 100%

Reference substance (positive control):

yes

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

20.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

12.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

14.9 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: nominal value corrected for average measured concentration of 74% of nominal

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

9.75 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

EC50 potassium dichromate 1-2 mg/L

Reported statistics and error estimates:

EC50 with Weibull analysis using linear max. likelihood regression

 Results of the test, 24 h values

	Control	6.25	12.5	25.0	50.0	100
		[mg/L]
	immobilised daphnids after 24 h
Group 1	0	0	0	1	2	5
Group 2	0	0	0	1	1	5
Group 3	0	0	0	0	4	5
Group 4	0	0	0	0	4	5
Σ	0	0	0	2	11	20
%	0	0	0	10	55	100

Results of the test, 48 h values

	Control	6.25	12.5	25.0	50.0	100
		[mg/L]
	immobilised daphnids after 48 h
Group 1	0	0	0	5	5	5
Group 2	0	0	0	4	5	5
Group 3	0	0	0	5	5	5
Group 4	0	0	0	4	5	5
Σ	0	0	0	18	20	20
%	0	0	0	90	100	100

Validity criteria fulfilled:

yes

Conclusions:

The EC50 of the substance is 14.9 mg/L (measured)

Executive summary:

In a test according to OECD 202, 20 daphnids per concentration (nominal: 0, 6.25, 12.5, 25.0, 50.0 and 100 mg/L, measured (geometric mean): ND, 4.75, 9.75, 20.3, 42.5 and 86.0 mg/L) were exposed for 48 hours. Immobility was observed at concentrations of 20.3 mg/L and higher. The EC50 of the substance is 20.1 mg/L (14.9 mg/L measured).

Description of key information

In a test according to OECD 202, 20 daphnids per concentration (nominal: 0, 6.25, 12.5, 25.0, 50.0 and 100 mg/L, measured (geometric mean): ND, 4.75, 9.75, 20.3, 42.5 and 86.0 mg/L) were exposed for 48 hours. Immobility was observed at concentrations of 20.3 mg/L and higher. The EC50 of the substance is 20.1 mg/L (14.9 mg/L measured).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

14.9 mg/L