4-ethoxy-2,3-difluoro-4'-propyl-1,1'-biphenyl

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

09.-10.01.2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Version / remarks:

2008

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Version / remarks:

2001

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Radiolabelling:

no

Test temperature:

25 °C

Details on study design: HPLC method:

EQUIPMENT
- Apparatus: HPLC
- Type, material and dimension of analytical (guard) column: LiChrospher 100 CN (250*4 mm, 5 μm)
- Detection system: UV-VIS

MOBILE PHASES
- Type: mobile phase: 55 % methanol / 45 % pure water; stationary phase: cyanopropyl
- Experiments with additives carried out on separate columns: no
- pH: not specified
- Solutes for dissolving test and reference substances: 55 % methanol / 45 % pure water

DETERMINATION OF DEAD TIME
- Method: by inert substances which are not retained by the column

REFERENCE SUBSTANCES
- Identity: methylbenzoate, pyrazophos, acetanilid, linuron, 1,2,3 trichlorobenzene, diclofop-methyl, sodium nitrate (inert substance)

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 10 µL solution with test item
- Quantity of reference substances: 10 µL solution with reference substances

REPETITIONS
- Number of determinations: 3

EVALUATION
- Calculation of capacity factors k': k' = (tR-t0)/t0
- Calculation of retention times: measured
- Determination of the log Koc value: The adsorption coefficient Koc was deduced from the capacity factor k’ using a calibration plot of log k’ versus log Koc of selected reference items.

Type:

log Koc

Value:

> 4.2 dimensionless

Temp.:

25 °C

Remarks on result:

other: pH not indicated

Details on results (HPLC method):

Please refer to attached document under "Overall remarks, attachments".

Validity criteria fulfilled:

yes

Conclusions:

The adsorption coefficient of the test substance was determined to be above 4.2 and estimated to be log Koc = 5.3.

Executive summary:

A study was conducted in accordance with OECD 121 and Commission Regulation (EC) No.440/2008, Annex, C.19 to determine the adsorption coefficient of the test substance using the HPLC method. The adsorption coefficient of the test substance was derived from its retention time and the calibration graph. The retention time of the test item was above the retention time of the highest calibration standard (log Koc 4.2) and thus out of the calibration range. Therefore, the adsorption coefficient of the test substance was determined to be above 4.2 and estimated to be log K_oc = 5.3.

Description of key information

The adsorption coefficient of the test substance was determined to be above 4.2 and estimated to be log K_oc = 5.3.

Additional information

A study was conducted in accordance with OECD 121 and Commission Regulation (EC) No.440/2008, Annex, C.19 to determine the adsorption coefficient of the test substance using the HPLC method. The adsorption coefficient of the test substance was derived from its retention time and the calibration graph. The retention time of the test item was above the retention time of the highest calibration standard (log Koc 4.2) and thus out of the calibration range. Therefore, the adsorption coefficient of the test substance was determined to be above 4.2 and estimated to be log K_oc = 5.3.