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EC number: 281-865-3 | CAS number: 84045-63-6
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1983
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Justification for type of information:
- None
- Qualifier:
- according to guideline
- Guideline:
- other: CIBA Method B-10
- Principles of method if other than guideline:
- None
- GLP compliance:
- no
- Specific details on test material used for the study:
- None
- Analytical monitoring:
- yes
- Details on sampling:
- None
- Vehicle:
- not specified
- Details on test solutions:
- None
- Test organisms (species):
- activated sludge
- Details on inoculum:
- None
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Remarks on exposure duration:
- None
- Post exposure observation period:
- None
- Hardness:
- None
- Test temperature:
- None
- pH:
- None
- Dissolved oxygen:
- None
- Salinity:
- None
- Conductivity:
- None
- Nominal and measured concentrations:
- None
- Details on test conditions:
- None
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 21 d
- Dose descriptor:
- IC50
- Effect conc.:
- > 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Details on results:
- None
- Results with reference substance (positive control):
- None
- Reported statistics and error estimates:
- None
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The IC50 for FAT 40000/D was found to be greater than 300 mg/l.
- Executive summary:
The toxicity of FAT 40000/D to activated sludge was determined in a 3-hours respiration inhibition test according to CIBA Method B-10. Based on the findings of the study, the IC50 of the test substance FAT 40000/D is greater than 300 mg/l.
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1984
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Justification for type of information:
- None
- Qualifier:
- according to guideline
- Guideline:
- other: CIBA Method B-10
- Principles of method if other than guideline:
- None
- GLP compliance:
- no
- Specific details on test material used for the study:
- None
- Analytical monitoring:
- yes
- Details on sampling:
- None
- Vehicle:
- not specified
- Details on test solutions:
- None
- Test organisms (species):
- activated sludge
- Details on inoculum:
- None
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Remarks on exposure duration:
- None
- Post exposure observation period:
- None
- Hardness:
- None
- Test temperature:
- None
- pH:
- None
- Dissolved oxygen:
- None
- Salinity:
- None
- Conductivity:
- None
- Nominal and measured concentrations:
- None
- Details on test conditions:
- None
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 21 d
- Dose descriptor:
- IC50
- Effect conc.:
- > 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Details on results:
- None
- Results with reference substance (positive control):
- None
- Reported statistics and error estimates:
- None
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The IC50 for FAT 40000/E was found to be greater than 300 mg/l.
- Executive summary:
The toxicity of FAT 40000/E to activated sludge was determined in a 21 days respiration inhibition test according to CIBA Method B-10. Based on the findings of the study, the IC50 of the test substance FAT 40000/E is greater than 300 mg/l.
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- None
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- 1. Deion. water instead of dest. water for the stocksolution 2. Preparation of the inoculum :higher sludge weight after the washing procedure (Sludge concentrations in the test according to the guidelines) 3. 4 instead of 5 test concentrations
- Principles of method if other than guideline:
- None
- GLP compliance:
- yes
- Specific details on test material used for the study:
- None
- Analytical monitoring:
- yes
- Details on sampling:
- None
- Vehicle:
- not specified
- Details on test solutions:
- None
- Test organisms (species):
- other: Activated sludge of a communal sewage treatment plant
- Details on inoculum:
- 3 times washing with tap water and addition of synthetic sewage (a day before the main test)
- Test type:
- other:
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- None
- Post exposure observation period:
- None
- Hardness:
- None
- Test temperature:
- 20.6 °C
- pH:
- 7.8 before the test beginning
- Dissolved oxygen:
- None
- Salinity:
- None
- Conductivity:
- None
- Details on test conditions:
- 320,100, 32 and 10 mg/l of the test substance
32,10 and 3.2 mg/l of the reference substance - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Details on results:
- None
- Results with reference substance (positive control):
- The IC50 for3,5- dichlorophenol was found to be 13.5 mg/L.
- Reported statistics and error estimates:
- None
- Validity criteria fulfilled:
- yes
- Conclusions:
- The IC50 for FAT 40000/G was found to be greater than 320 mg/L.
- Executive summary:
The toxicity of FAT 40000/G to activated sludge was determined in a 3-hours respiration inhibition test according to the OECD Guideline No. 209, and to the EG Method Nr.L 133/118. The test substance concentrations tested were 320,100, 32 and 10 mg/l. The reference substance 3,5-Dichlorophenol was tested in concentrations of 32,10 and 3.2 mg/l. The IC50 for reference substance was found to be 13.5 mg/L. Based on the findings, the IC50 for FAT 40000/G was found to be greater than 320 mg/L
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
1. HYPOTHESIS FOR THE ANALOGUE APPROACH
This read-across is based on the hypothesis that the source and the target substance have very similar physicochemical and (eco)toxicological properties because their chemical structures are nearly identical. An analogue approach has thus been employed. The target substance is the meta-isomer of the dye Reactive Yellow 095, where the sulphonate group is bound at the meta-position of the amino benzene moiety. The source chemical is the reaction mass of both the meta-isomer and the para-isomer of Reactive Yellow 095.
The presence of sulphonate groups make both dyes highly water soluble and therefore less critical for human health and environmental issues. Based on their chemical similarity, similar properties are expected in both humans and the environment.
2. SOURCE AND TARGET CHEMICAL(S)
Source: Reactive Yellow 095 meta/para (CAS# --- / EC# 944-218-2)
Target: Reactive Yellow 095 meta (CAS# 84045-63-6 / EC# 281-865-3)
3. ANALOGUE APPROACH JUSTIFICATION
see attachment under 4.12 Auto flammability - Reason / purpose for cross-reference:
- read-across source
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 3h-IC50 in microorganisms (activated sludge) was determined to be >320 mg/L.
- Executive summary:
The toxicity of the source substance to activated sludge was determined in a 3-hours respiration inhibition test according to the OECD Guideline No. 209. The test substance concentrations tested were 320,100, 32 and 10 mg/l. The reference substance 3,5-Dichlorophenol was tested in concentrations of 32,10 and 3.2 mg/l. The IC50 for the reference substance was found to be 13.5 mg/L. Based on the findings, the IC50 for the source substance was found to be greater than 320 mg/L
The structurally related target substance will show the same behaviour and therefore it is anticipated that the 3h-IC50 will also be >320 mg/L.
Referenceopen allclose all
None
None
None
Description of key information
The 3h-IC50 in microorganisms (activated sludge) was determined to be greater than 320 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 320 mg/L
- EC10 or NOEC for microorganisms:
- 320 mg/L
Additional information
The toxicity of the source substance to activated sludge was determined in a 3-hour respiration inhibition test according to OECD Guideline 209. Based on the findings of the study, the IC50 of the source substance is greater than 320 mg/L. Two supporting studies involving different batches of the source substance have been conducted. Similar results were achieved.
The structurally related target substance will show the same behaviour and therefore it is anticipated that the 3h-IC50 will also be >320 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
