Trisodium 5-[[5-[[4-chloro-6-[(3-sulphonatophenyl)amino]-1,3,5-triazin-2-yl]amino]-2-sulphonatophenyl]azo]-1-ethyl-1,2-dihydro-6-hydroxy-4-methyl-2-oxopyridine-3-methanesulphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1973

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Justification for type of information:

None

Qualifier:

according to guideline

Guideline:

other: CIBA Method A-11

Principles of method if other than guideline:

None

GLP compliance:

no

Specific details on test material used for the study:

None

Analytical monitoring:

yes

Details on sampling:

None

Vehicle:

yes

Details on test solutions:

Water

Test organisms (species):

other: trout and goldorfe

Details on test organisms:

None

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Remarks on exposure duration:

None

Post exposure observation period:

None

Hardness:

None

Test temperature:

None

pH:

None

Dissolved oxygen:

None

Salinity:

None

Conductivity:

None

Nominal and measured concentrations:

None

Details on test conditions:

None

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC0

Remarks:

TL0

Effect conc.:

200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks:

trout

Duration:

96 h

Dose descriptor:

LC0

Remarks:

TL0

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks:

goldorfe

Key result

Duration:

96 h

Dose descriptor:

LC50

Remarks:

TL50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Remarks:

TL100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

None

Results with reference substance (positive control):

None

Reported statistics and error estimates:

None

Sublethal observations / clinical signs:

None

Validity criteria fulfilled:

not specified

Conclusions:

The LC50 (TL) of FAT 40000 in this fish acute toxicity test was determined to be greater than 1000 mg/L.

Executive summary:

An acute fish toxicity test was performed with trout and goldorfe. Groups of fish were exposed at the test concentrationsof 100, 200 and 1000 mg/L. Based on the findings, the LC0 value was 200 mg/l (trout) or 1000 mg/l (goldorfe), whereas LC50 and LC100 value was determined to be greater than 1000 mg/l in both species.

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1977

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Justification for type of information:

None

Qualifier:

according to guideline

Guideline:

other: CIBA Method A-11

Principles of method if other than guideline:

None

GLP compliance:

no

Specific details on test material used for the study:

None

Analytical monitoring:

yes

Details on sampling:

None

Vehicle:

yes

Details on test solutions:

Water

Test organisms (species):

Salmo sp.

Details on test organisms:

None

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Remarks on exposure duration:

None

Post exposure observation period:

None

Hardness:

None

Test temperature:

12 °C

pH:

8

Dissolved oxygen:

None

Salinity:

None

Conductivity:

None

Nominal and measured concentrations:

100, 200 and 1000 mg/l

Details on test conditions:

None

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC0

Remarks:

TL0

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Remarks:

TL50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Remarks:

TL100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

None

Results with reference substance (positive control):

None

Reported statistics and error estimates:

None

Sublethal observations / clinical signs:

None

Validity criteria fulfilled:

not specified

Conclusions:

The LC50 (TL) of FAT 40000/A in this fish acute toxicity test was determined to be greater than 1000 mg/L.

Executive summary:

An acute fish toxicity test was performed with trout. Groups of fish were exposed at the test concentrationsof 100, 200 and 1000 mg/L. Based on the findings, the LC0, LC50 and LC100 (TL) value was determined to be greater than 1000 mg/l.

Reference 3

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1980

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Justification for type of information:

None

Qualifier:

according to guideline

Guideline:

other: CIBA Method A-11

Principles of method if other than guideline:

None

GLP compliance:

no

Specific details on test material used for the study:

None

Analytical monitoring:

yes

Details on sampling:

None

Vehicle:

yes

Details on test solutions:

Water

Test organisms (species):

other: Red killi and trout

Details on test organisms:

None

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Remarks on exposure duration:

None

Post exposure observation period:

None

Hardness:

None

Test temperature:

None

pH:

None

Dissolved oxygen:

None

Salinity:

None

Conductivity:

None

Nominal and measured concentrations:

None

Details on test conditions:

None

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC0

Remarks:

TL0

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Remarks:

TL50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Remarks:

TL100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

None

Results with reference substance (positive control):

None

Reported statistics and error estimates:

None

Sublethal observations / clinical signs:

None

Validity criteria fulfilled:

not specified

Conclusions:

The LC50 (TL) of FAT 40000/C in this fish acute toxicity test was determined to be greater than 1000 mg/L.

Executive summary:

An acute fish toxicity test was performed with Red killi fish and trout. Based on the findings, the LC0, LC50 and LC100 (TL) value was determined to be greater than 1000 mg/l for both fish species.

Reference 4

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1983

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Justification for type of information:

None

Qualifier:

according to guideline

Guideline:

other: CIBA Method A-11

Principles of method if other than guideline:

None

GLP compliance:

no

Specific details on test material used for the study:

None

Analytical monitoring:

yes

Details on sampling:

None

Vehicle:

yes

Details on test solutions:

Water

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

None

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Remarks on exposure duration:

None

Post exposure observation period:

None

Hardness:

None

Test temperature:

25 °C

pH:

10

Dissolved oxygen:

None

Salinity:

None

Conductivity:

None

Nominal and measured concentrations:

300 and 1000 mg/l

Details on test conditions:

None

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

None

Results with reference substance (positive control):

None

Reported statistics and error estimates:

None

Sublethal observations / clinical signs:

None

Validity criteria fulfilled:

not specified

Conclusions:

The LC50 of FAT 40000/D in this fish acute toxicity test was determined to be greater than 1000 mg/L.

Executive summary:

An acute fish toxicity test was performed with Zebra-fish ( Brachydanio rerio ). Groups of fish were exposed at the test concentrationsof 300 and 1000 mg/L. Based on the findings, the LC0, LC50 and LC100 value was determined to be greater than 1000 mg/l.

Reference 5

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1984

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Justification for type of information:

None

Qualifier:

according to guideline

Guideline:

other: CIBA Method A-11

Principles of method if other than guideline:

None

GLP compliance:

no

Specific details on test material used for the study:

None

Analytical monitoring:

yes

Details on sampling:

None

Vehicle:

yes

Details on test solutions:

Water

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

None

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Remarks on exposure duration:

None

Post exposure observation period:

None

Hardness:

None

Test temperature:

23 °C

pH:

10

Dissolved oxygen:

None

Salinity:

None

Conductivity:

None

Nominal and measured concentrations:

300 and 1000 mg/l

Details on test conditions:

None

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

300 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

None

Results with reference substance (positive control):

None

Reported statistics and error estimates:

None

Sublethal observations / clinical signs:

None

Validity criteria fulfilled:

not specified

Conclusions:

The LC50 of FAT 40000/E in this fish acute toxicity test was determined to be greater than 1000 mg/L.

Executive summary:

An acute fish toxicity test was performed with Zebra-fish (Brachydanio rerio). Groups of fish were exposed at the test concentrationsof 300 and 1000 mg/L. Based on the findings, the LC0 value was determined to be 300 mg/l. The LC50 and LC100 value was determined to be greater than 1000 mg/l.

Reference 6

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

REPORTING FORMAT FOR THE ANALOGUE APPROACH
1. HYPOTHESIS FOR THE ANALOGUE APPROACH
This read-across is based on the hypothesis that the source and the target substance have very similar physicochemical and (eco)toxicological properties because their chemical structures are nearly identical. An analogue approach has thus been employed. The target substance is the meta-isomer of the dye Reactive Yellow 095, where the sulphonate group is bound at the meta-position of the amino benzene moiety. The source chemical is the reaction mass of both the meta-isomer and the para-isomer of Reactive Yellow 095.
The presence of sulphonate groups make both dyes highly water soluble and therefore less critical for human health and environmental issues. Based on their chemical similarity, similar properties are expected in both humans and the environment.
2. SOURCE AND TARGET CHEMICAL(S)
Source: Reactive Yellow 095 meta/para (CAS# --- / EC# 944-218-2)
Target: Reactive Yellow 095 meta (CAS# 84045-63-6 / EC# 281-865-3)
3. ANALOGUE APPROACH JUSTIFICATION
see attachment under 4.12 Auto flammability

Reason / purpose for cross-reference:

read-across source

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

not specified

Conclusions:

The 96h-LC50 in zebrafish was >1000 mg/L.

Executive summary:

Based on one key study and four supporting studies for the source substance, it was clear that the 96h-LC50 in fish was >1000 mg/L. The structurally related target substance will show the same behaviour, and the 96h-LC50 will be as well >1000 mg/L.

Description of key information

The LC50 in fish was determined to be greater than 1000 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 000 mg/L

Additional information

A key study to determine acute toxicity to fish was performed with Zebra-fish (Brachydanio rerio). Groups of fish were exposed at the test concentrations of 300 and 1000 mg/L. Based on the findings, the LC0 values was 300 mg/L, whereas LC50 and LC100 value was determined to be greater than 1000 mg/L. Several supporting studies conducted on different batches of this test substance and involving different fish species confirmed this test result indicating that the effects are not dependent on fish species or production batch.

The structurally related target substance will show the same behaviour and therefore it can be anticipated that the 96h-LC50 in fish will be >1000 mg/L as well.