Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 944-296-8 | CAS number: -
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- other: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- From August 21 to August 25, 1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- DIN 38 412 part 15 (1982)
- GLP compliance:
- no
- Test organisms (species):
- Leuciscus idus
- Details on test organisms:
- TEST ORGANISM
- Common name: golden orfe.
- Source: Fischzucht Paul Eggers.
- Length at study initiation: 7.2 cm (range: 6.2 - 7.9).
- Weight at study initiation: 3.5 g (range: 2.4 - 4.4).
- Housing: fish were kept in a flow-through tank in tap water cleaned by active carbon and aerated with oil-free air.
- Feeding during test: no, withdrawal of food one day before and during exposure.
- Food type: growing feed F/B 50.
- Amount: ad libitum.
ACCLIMATION
- Duration of housing and adaptation: 6 months.
- Adaptation to test water and test temperature: 3 days.
- Temperature: 20 ± 1°C.
- Photoperiod: 16 hours light and 8 hours dark. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 2.5 mmol/l
- Test temperature:
- 20 ± 1°C
- pH:
- ca 8.0
- Dissolved oxygen:
- 7.5 - 8.6 mg/l
- Conductivity:
- max 10 micromho
- Nominal and measured concentrations:
- 0, 46.4, 100, 215, 464, 1000 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 10 L glass aquarium (30 cm × 22 cm × 24 cm).
- Aeration: continuous aeration with oil-free air.
- No. of organisms per vessel: 10.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38 412, part 11, October 1982.
- Ca/mg ratio: 4:1.
- Na/K ratio: 10:1.
- Conductivity: 10 µS.
OTHER TEST CONDITIONS
- pH: 8.
- Photoperiod: 16 hours of light and 8 dark.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2.
- Range finding study: yes.
- Test concentrations: 46.4, 100, 215, 464 and 1000 mg/l. - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 464 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- ca. 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 215 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- symptoms: apathy, tumbling, narcotic state.
- Sublethal observations / clinical signs:
Oxygen content mg/L:
nominal conc. mg/L 1 h 4 h 24 h 48 h 72 h 96 h 0 7.0 7.5 7.8 7.6 8.6 46.4 7.6 8.0 8.1 8.2 8.0 100 7.7 8.3 8.1 8.2 8.4 215 7.6 8.3 8.4 8.5 8.6 464 7.2 8.0 8.0 8.1 8.6 1000 7.4 8.2 8.0 8.1 8.6 pH:
nominal conc. mg/L 1 h 4 h 24 h 48 h 72 h 96 h 0 7.3 7.2 7.3 7.4 7.4 46.4 7.2 7.2 7.3 7.4 7.2 100 7.2 7.3 7.3 7.4 7.3 215 7.1 7.3 7.2 7.4 7.2 464 7.0 7.2 7.1 7.3 7.3 1000 6.7 7.0 7.3 7.2 7.3 Dead fish after:
nominal conc. mg/L no. of fish 1 h 4 h 24 h 48 h 72 h 96 h 0 10 0 0 0 0 0 0 46.4 10 0 0 0 0 0 0 100 10 0 0 0 0 0 0 215 10 0 0 0 0 0 0 464 10 0 0 0 0 0 0 1000 10 0 0 0 0 0 0 - Validity criteria fulfilled:
- yes
- Conclusions:
- LC50 is ca. 1000 mg/L.
- Executive summary:
Method:
The substance was tested for short-term toxicity on fish according to the guideline DIN 38 412 part 15. Tested concentrations were 0, 46.4, 100, 215, 464, and 1000 mg/L for 96 hours, using 10 fish per concentration. Observations were done 1, 4, 24, 48, 72 and 96 h after start of exposure.
Results:
NOEC = 215 mg/L
LC0 = 464 mg/L
LC50 ca. 1000 mg/L
LC100 > 1000 mg/L
During test period, the glass aquarium was intensely coloured and cloudy increasing with an increase in the concentration. The symptoms observed were apathy, tumbling, narcotic state.
Reference
Description of key information
LC50 (96 h) ca. 1000 mg/L
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 1 000 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
