Disodium hydrogen bis[4-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-3-hydroxynaphthalene-1-sulphonato(3-)]chromate(3-)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

other: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Study period:

From September 5th to 9th, 1995.

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

abstract

Remarks:

Test conducted according to internationally accepted testing guidelines, nevertheless only a summary is available. Justification for Read Across is detailed in the endpoint summary.

Principles of method if other than guideline:

Acute toxicity of substance to invertebrates was investigated in a 48 hours: 5 animals per vessel and two vessels per test substance concentration were used (0.1, 1.0, 10 and 100 mg/l); two vessels were used as vehicle control and further two vessels as blank control.

GLP compliance:

no

Details on test solutions:

Concentration: 0.050 g/0.5 l
Vehicle: 5 mg/l

Test organisms (species):

Daphnia sp.

Test type:

not specified

Water media type:

not specified

Limit test:

no

Total exposure duration:

48 h

Test temperature:

21 - 22 °C

pH:

7.5 - 7.9

Dissolved oxygen:

96 - 98 %

Nominal and measured concentrations:

Nominal concentrations: 0.1, 1.0, 10 and 100 mg/l

Details on test conditions:

TEST SYSTEM
- No. of organisms per vessels: 5
- No. of vessels per concentration: 2
- No. of vessels per control: 2
- No. of vessels per vehicle control: 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Nominal concentration (mg/l)	No animals	No. of immobilized Daphnia		% immobility		pH at the end	O2 (%) at the end
		24-h	48-h	24-h	48-h
Vehicle	5	0	0	0	0	7.9	98
	5	0	0			7.9	98
Blank	5	0	0	0	0	7.9	98
	5	0	0			7.9	98
0.1	5	0	0	0	0	7.8	97
	5	0	0			7.8	97
1	5	0	0	0	0	7.8	97
	5	0	0			7.8	97
10	5	0	0	0	0	7.8	96
	5	0	0			7.8	97
100	5	0	0	0	0	7.8	96
	5	0	0			7.8	96

Validity criteria fulfilled:

yes

Remarks:

in the control not more that 10 % of the daphnids were immobilised and the dissolved oxygen concentration at the end of the test was 3 mg/l in control and test vessels

Conclusions:

EC50 (48h) > 100 mg/l (nominal)

Executive summary:

Acute toxicity of substance to invertebrates was investigated in a 48 hours: 5 animals per vessel and two vessels per test substance concentration were used (0.1, 1.0, 10 and 100 mg/l); two vessels were used as vehicle control and further two vessels as blank control.

Conclusion

EC50 (48h) > 100 mg/l (nominal)

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

other: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

abstract

Remarks:

Test conducted according to internationally accepted testing guidelines, nevertheless only a summary is available. Justification for Read Across is detailed in the endpoint summary.

Principles of method if other than guideline:

Acute toxicity of substance to Daphnia Magna was investigated in a 48 hours screening test. 5 animals per vessel and two vessels per test substance concentration were used (0.1, 1.0, 10 and 100 mg/l); two vessels were used as vehicle control and further two vessels as blank control.

GLP compliance:

no

Test organisms (species):

Daphnia magna

Test type:

not specified

Water media type:

not specified

Limit test:

no

Total exposure duration:

48 h

Test temperature:

21 - 22 °C

pH:

7.5 - 7.9

Dissolved oxygen:

96 - 98 %

Nominal and measured concentrations:

Nominal concentrations: 0.1, 1.0, 10 and 100 mg/l

Details on test conditions:

TEST SYSTEM
- No. of organisms per vessels: 5
- No. of vessels per concentration: 2
- No. of vessels per control: 2
- No. of vessels per vehicle control: 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

EC0 at 48 hours: 100 mg/l

Immobilization of Daphnia: number of immobilized Daphnia after 24 and 48 hours

Nominal concentration (mg/l)	No animals	No. of immobilized Daphnia		% immobility
		24-h	48-h	24-h	48-h
Vehicle	5	0	0	0	0
	5	0	0
Blank	5	0	0	0	0
	5	0	0
0.1	5	0	0	0	0
	5	0	0
1	5	0	0	0	0
	5	0	0
10	5	0	0	0	0
	5	0	0
100	5	0	0	0	0
	5	0	0

Oxygen, pH and temperature

Nominal conc. (mg/l)	Start	End
	100	Vehicle	Blank	0.1	1	10	100
Oxygen (%)	satured	98	98	97	97	97	96
pH	7.5	7.9	7.9	7.8	7.8	7.8	7.8
Temperature (°C)	22	21	21	21	21	21	21

Validity criteria fulfilled:

yes

Remarks:

in the control not more that 10 % of the daphnids were immobilised and the dissolved oxygen concentration at the end of the test was 3 mg/l in control and test vessels

Conclusions:

EC50 (48h) > 100 mg/l (nominal)

Executive summary:

Acute toxicity of substance to Daphnia Magna was investigated in a 48 hours screening test. 5 animals per vessel and two vessels per test substance concentration were used (0.1, 1.0, 10 and 100 mg/l); two vessels were used as vehicle control and further two vessels as blank control.

Conclusion

EC50 (48h) > 100 mg/l (nominal)

Description of key information

EC50 > 100 mg/l