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EC number: 262-134-8 | CAS number: 60270-33-9
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
LC50 = 5.45 mg/L, analysed test concentrations of the untreated solution (OECD Guideline 203, semi-static; GLP, RL1)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 5.45 mg/L
Additional information
In a 96-h acute toxicity study according toOECD guideline 203 (Fish, Acute Toxicity Test) 1992 and EU Method C.1 (Acute Toxicity for Fish) 1992, juvenile rainbow trouts (Oncorhynchus mykiss) were exposed to DIMAPDO lactate at nominal concentrations of 1.0, 1.8, 3.2, 5.6 and 10 mg test substance/L (mean measured concentrations of untreated test medium: 0.63, 1.5, 2.9, 4.8 and 9.1 mg/L; mean measured concentrations of centrifuged test medium: 0.32, 0,76, 1.2, 2.6 and 6.6 mg/L) under semi-static conditions. The 96 -hour LC50 based on nominal test concentrations was 7.5 mg/L with 95% confidence limits of 5.6 - 10 mg/L. The 96 -hour LC50 based on mean measured concentrations of untreated test medium was 6.6 mg/L with 95% confidence limits of 4.8 - 9.1 mg/L.
The analysed test concentrations of the untreated solution (analysis of dispersed + dissolved test substance) and not of the centrifuged solution (analysis only of dissolved test substance) were chosen for further risk assessment because in the OECD guideline 201 study there was a discontinuity in the analysis of the concentrations of the centrifuged test solution and therefore the calculated values for the centrifuged test media were not valid. Additionally the examination of the dead fish revealed that the dispersed test material had adhered to the gill filaments indicating physical toxicity of the dispersed test material particles may be a factor in toxicity to fish.
Because the study was conducted with the lactic acid salt of DIMAPDO, the 96-h LC50 of DIMAPDO based on mortality and sublethal effects and the concentration analysis of the untreated test solution were calculated as 5.45 mg/L active ingredient.
Sub-lethal effects (moribundity) of exposure were observed at a nominal test concentration of 10 mg/L. Due to the approach of substantial severity limit these fish were killed and classed as mortalities at 24 and 48-hour time points respectively.
This toxicity study is classified as acceptable and satisfies the guideline requirement for a fish short-term toxicity study.
Results Synopsis
Test organism size/age: juvenile rainbow trouts (Oncorhynchus mykiss); mean length: 4.2 cm (SD = 0.1); weight: 0.90 g (SD = 0.21)
Test Type: semi-static (daily renewal)
LC50: 5.45 mg a.i./L 95% C.I.: 3.96 to 7.51 mg a.i./L
NOEC: 3.96 mg a.i./L
Endpoint(s) Effected: mortality
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