Hydrazine

Basic toxicokinetics

Administrative data

Endpoint:

basic toxicokinetics in vivo

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: report in brief of different application routes

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1986

Materials and methods

Objective of study:

toxicokinetics

Principles of method if other than guideline:

Administration of test substance via gavage, drinking water, oral undefined, subcutan, intratracheal and dermal without and with collar and availabiltiy in blood and excretion rate were determined.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

hydrazine hydrate

Cas Number:

10217-52-4

Molecular formula:

H4N2*xH2O

IUPAC Name:

hydrazine hydrate

Constituent 2

Reference substance name:

hydrazine hydrate

IUPAC Name:

hydrazine hydrate

Details on test material:

hydrazine hydrate (99 %)

Radiolabelling:

no

Test animals

Species:

rat

Strain:

other: CPB-SPF

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 180-220 g
- metabolism cages: yes, only animals that were nominated for urinalysis

Administration / exposure

Route of administration:

other: (1) oral gavage, (2) drinking water, (3) oral undefined, (4) subcutan, (5) intratracheal, (6) dermal

Vehicle:

water

Details on exposure:

see section additional information on material and methods

Duration and frequency of treatment / exposure:

see section additional information on material and method

Doses / concentrations

Remarks:

Doses / Concentrations:
see section additional information on material and method

No. of animals per sex per dose / concentration:

see section additional information on material and method

Control animals:

yes

Positive control reference chemical:

no

Details on study design:

determination of hydrazine in blood:
Reaction of hydrazine with p-dimethylaminobenzaldehyd result in
Dimethylaminobenzalazin (DMABA) which is yellow and
can be extracted with chloroform and measured fluorimetrically
urinary analysis
by HPLC combined with UV-detection

Details on dosing and sampling:

see secton addional information on material and methods

Statistics:

no data

Results and discussion

Preliminary studies:

no data

Toxicokinetic / pharmacokinetic studies

Details on absorption:

see section remarks on result

Details on distribution in tissues:

no data

Transfer into organs

Transfer type:

other: not determened

Observation:

other: no data

Details on excretion:

see section remarks on result

Toxicokinetic parameters

Toxicokinetic parameters:

other: see section remarks on results

Metabolite characterisation studies

Metabolites identified:

no

Details on metabolites:

no data

Any other information on results incl. tables

Trial (1) blood examination: rapid absorption from the gastrointestinal tract; highest concentration 15 min post application: 234 ng/ml and 441 ng/ml, respectively half live in blood: 35 min and 90 min., respectively urinalysis within 24 hours 13.1 and 16.9 % of the dose as unchanged hydrazine trial(2) blood examination 10 ppm: 13.6 ng/ml 50 ppm: 48 ng/ml urinalysis 10 ppm: 216 µmol 50 ppm: 715 µmol trial(3)-(6) (3) oral unspecified: 23.4-27.5 % of the dose (4) subcutan: 37.2-50.9 % of the dose (5) intratracheal: 28.3 % of the dose (6a) dermal (without collar) 21.1 % of the dose (6b) dermal (with collar) 14.7-7.5 % of the dose.