Calcium bromide

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1978

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

no guideline available

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Calcium bromide

EC Number:

232-164-6

EC Name:

Calcium bromide

Cas Number:

7789-41-5

Molecular formula:

CaBr2

IUPAC Name:

calcium bromide

Details on test material:

The test material was received from the Great Lakes Chemical Corporation, West Lafayette, Indiana on April 29, 1977. It was identified as "Calcium Bromide (solid), (417-130B), H1510ff and was received as a white powder.

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Details on test animals or test system and environmental conditions:

Four male and 4 female Kew Zealand White rabbits were used for this study. The rabbits weighed from 2304 to 2934 grams at the beginning of this study. They were individually housed in hanging wire-mesh cages in temperature and humidity controlled quarters. They were maintained in accordance with the recommendations contained in H.E.W. Publication No. 74-23 (N.I.H.) entitled "Guide for the Care and Use of Laboratory Animals". Water and Purina Rabbit Chow were available ad libitum. Body weights were measured initially (control weight) and at 14 days.

Administration / exposure

Type of coverage:

occlusive

Vehicle:

physiological saline

Details on dermal exposure:

TEST SITE
- Area of exposure: back
- % coverage: 20-30% of the body surface
- Type of wrap if used: The area of application was wrapped wirh gauze bandaging and occluded with Saran Wrap

REMOVAL OF TEST SUBSTANCE
- Washing (if done): Twenty-four hours later the bandages were removed and the backs were washed with tepid tap water.
- Time after start of exposure: 24 hours

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 200 and 2000 mg/kg

Duration of exposure:

24 hours

Doses:

200 and 2000 mg/kg

No. of animals per sex per dose:

2

Control animals:

no

Details on study design:

The hair was removed from the back of each rabbit (20 - 30% of the body surface) with an electric clipper. The rabbits were divided into 2 groups of 2 male and 2 female rabbits each. The skin of one male and one female in each group was abraded with a scalpel blade. The abrasions penetrated the stratum corneum, but were not deep enough to cause bleeding. The test material was applied once only to the backs of the rabbits, using 0.9% physiological saline as the wetting agent, at the following dosage levels: 200 and 2000 mg/kg. The area of application was wrapped wirh gauze bandaging and occluded with Saran Wrap. Twenty-four hours later the bandages were removed and the backs were washed with tepid tap water. They vere observed at 24 hours and daily thereafter for a total of 14 days for mortality only.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

other: minimum lethal dose

Effect level:

> 200 - < 2 000 mg/kg bw

Based on:

test mat.

Remarks:

CaBr2

Mortality:

One of 4 rabbits at the 2000 mg/kg dosage level died. None of the rabbits at the 200 mg/kg dosage level died during the observation period.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: not specified

Conclusions:

The minimum lethal dose by the dermal route of administration was found to be less than 2000 mg/kg but greater than 200 mg/kg.
In accordance with the requirements of the Federal Hazardous Substances act this test material would not be considered a toxic substance by the dermal route of administration.