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Diss Factsheets

Administrative data

Endpoint:
biodegradation in soil
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study conducted in accordance with GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 307 (Aerobic and Anaerobic Transformation in Soil)
GLP compliance:
yes
Test type:
laboratory

Test material

Constituent 1
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 945-0101

RADIOLABELLING INFORMATION (if applicable)
- Radiochemical purity: 99.8%
- Specific activity:12 MBq/mg (720000 dpm/ug)
- Locations of the label: pyrazole-4-1 4C
Radiolabelling:
yes

Study design

Oxygen conditions:
aerobic
Soil classification:
USDA (US Department of Agriculture)
Soil propertiesopen allclose all
Soil no.:
#1
Soil type:
loamy sand
% Clay:
3.5
% Silt:
12
% Sand:
84.5
% Org. C:
0.99
pH:
7
CEC:
5.5 meq/100 g soil d.w.
Soil no.:
#2
Soil type:
sand
% Clay:
3.1
% Silt:
9.3
% Sand:
87.6
% Org. C:
1.73
pH:
6.5
CEC:
7.2 meq/100 g soil d.w.
Soil no.:
#3
Soil type:
loamy sand
% Clay:
5
% Silt:
13
% Sand:
82
% Org. C:
2.6
pH:
6.4
CEC:
15.3
Duration of test (contact time)
Duration:
120 d
Initial test substance concentration
Initial conc.:
0.27 mg/kg soil d.w.
Based on:
test mat.
Experimental conditions
Temp.:
20 °C
Humidity:
40% of the maximum water holding capacity
Details on experimental conditions:
EXPERIMENTAL DESIGN
- Details of traps for CO2 and organic volatile, if any: 1. trap: 0.5 mol/L NaOH, 2. trap: 0.5 mol/L H2SO4, 3. trap: ethylene glycol
- Temperature: 20 °C

Test material application
- Volume of test solution used/treatment: 100 g soil

Experimental conditions (in addition to defined fields)
- Continuous darkness: Yes

SAMPLING DETAILS
- Sampling intervals: 0, 3, 7, 14, 28/30, 58/63, 90/91, and 120 days

Results and discussion

Material (mass) balanceopen allclose all
% Degradation
% Degr.:
1.1
Parameter:
radiochem. meas.
Sampling time:
63 d
Half-life / dissipation time of parent compoundopen allclose all
Soil No.:
#1
DT50:
9.3 d
Type:
(pseudo-)first order (= half-life)
Temp.:
20 °C
Soil No.:
#2
DT50:
6.5 d
Type:
(pseudo-)first order (= half-life)
Temp.:
20 °C
Soil No.:
#3
DT50:
2.3 d
Type:
other: FOMC
Temp.:
20 °C
Details on transformation products:

In all three soils only one metabolite was formed in significant amounts representing
metabolite 3-(difluoromethyl)-1H-pyrazole-4-carboxylic acid as was confirmed by mass spectrometry (LC-MS/MS). 3-(difluoromethyl)-1H-pyrazole-4-carboxylic acid
already appeared within 3 days of incubation. It quickly increased to maximum amounts of
67.4-76.0% TAR within 30 days, followed by a constant decrease to 44.3-59.2% TAR at the
end of the incubation period.

Applicant's summary and conclusion