m-tolyl isocyanate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Principles of method if other than guideline:

Method: 14-day LC50 study was conducted as described by Könemann (Quantitative structure-acitvity relationships in fish toxicity studies. I. Relationship for 50 industrial polluants. Toxicology 19, 209-221, 1981)

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

yes

Details on test solutions:

- stock solutions of the test substance were prepared in 2-propanol

Test organisms (species):

Poecilia reticulata

Details on test organisms:

- Age at study initiation: 2-3 months

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

14 d

Hardness:

25 mg/L CaCO3

Test temperature:

22+1°C

Dissolved oxygen:

>5 mg/L

Details on test conditions:

- 1.5 L vessels, filled with 1L test solution and coverd with glass
- Renewal rate of test solution: daily
- No. of organisms per concentration: 8 fish
- using standard water, prepared according to Alabaster and Abram, 1964
- fish were feed 0.5 h before renewal test solution
- maximal 100 µL of the stock solution were added to 1 L of the standard water

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

36.3 mg/L

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

not specified

Remarks:

Since the test is poorly reported the validity criteria cannot be assessed sufficiently. Nevertheless the test is considered as valid based on the data given.

Conclusions:

For the substance a 14d-LC50 of 36.3 mg/L was determined on fish (Poecilia reticulata).

Executive summary:

The short-term toxicity of the substance towards fish was performed in a semi static test system which was in most parts equivalent to the OECD guideline 204 (Fish, prolonged toxicity test), a 14 d-LC50 of 36.31 mg/L was determined.

Description of key information

14-d LC50 = 36.3 mg/L (Hermens, 1984)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

36.3 mg/L

Additional information

Due to rapid hydrolysis of isocynantes, the acute toxicity of the primary amine transformation product of m-tolyl isocyanate, m-toluidine, to fish is presented as the most relevant endpoint for use in the CSA.

The short-term toxicity of the substance towards fish was performed in a semi static test system which was in most parts equivalent to the OECD guideline 204 (Fish, prolonged toxicity test), a 14 d-LC50 of 36.31 mg/L was determine (Hermens, 1984).