Amides, vegetable-oil, N,N-bis(hydroxyethyl)

Endpoint summary

Additional information

Acute data

Fish

Amides, C8-18 (even numbered) and C18-unsatd., N,N-bis(hydroxyethyl), CAS 68155-07-7 was tested according to the OECD Guideline 203 on Oncorhynchus mykiss (Rainbow trout) in a semi-static condition, for 96 hours.

LC50 based on mortality is resulted to be 2.4 mg/L, therefore this similar substance can be considered to be toxic to rainbow trout.¹

Amides, C16-18 (even-numbered) and C18 (unsatd.), N,N-bis(hydroxyethyl), CAS 68603-38-3, was tested according to the OECD Guideline 203 on Rainbow trout. Mortality and other sub-lethal effects such swimming at the bottom and surface, loss of equilibrium and moribundity were recorded at 3, 6, 24, 48, 72 and 96 h. The LC50 resulted is 1.2 mg/L.

A study was also conducted to assess the acute toxicity of the same amide in Zebra-fish according to ISO-guideline 7346/2 (conforms to OECD guideline 203). Under the test conditions, the test substance was found to be toxic to Zebra fish with an LC50 value of 5.6 mg/L (based on nominal concentration) i.e., equivalent to 4.9 mg active matter/L.

An additional test on Coconut fatty acid diethanolamide, CAS 68603-42-9 can be considered. It was performed according to the OECD Guideline 203 on Brachydanio rerio (Danio rerio) in a semi-static condition, for 96 hours, revaling toxicity. The LC50 resulted is 4.9 mg/L, that revealed acute toxicity on fish.^{1 3}

Aquatic invertebrates

Amides, C12-18 (even-numbered) and C18 (unsatd.), N,N-bis(hydroxyethyl), CAS 90622-74-5 was tested according to OECD 202 on Daphnia magna for 24 hours in a static condition.

The EC50 resulted 3.3 mg/L therefore this similar substance was found to be toxic to Daphnia magna.²

A test on Amides, soya, N,N-bis(hydroxyethyl), CAS 68425 -47-8 was performed according to the OECD Guideline 202 on Daphnia magna in a static condition, for 48 hours. Under the study conditions, the 48h EC50 was low, 0.9 mg/L (nominal).³

Additional test on Coconut fatty acid diethanolamide, CAS 68603-42-9 can be considered. Under the test condition, the LC50 at 48 hours on Daphnia magna resulted 3.2 mg/L, revealing toxicity on aquatic invertebrates.^{1 2 3}

Algae

Amides, C12-18 (even-numbered) and C18 (unsatd.), N,N-bis(hydroxyethyl), CAS 90622-74 -5, was tested according to the OECD Guideline 201 on in a Scenedesmus subspicatus (new name: Desmodesmus subspicatus), for 72 hours. Under the test conditions, the EbC50 and ErC50 were estimated to be 2.2 and 7.4 mg/L, respectively.

In a similar study on the same similar substance, the EbC50 and ErC50 at 72 h were estimated to be 2.1 and 3.9 mg/L, respectively.²

A study on the similar soya-derivative substance CAS 68425-47-8, is available.

Under the conditions of this study, the 72h ErC50 of the test substance for Scenedesmus subspicatus, based on nominal concentrations, was 18.6 mg/L and the 72h EbC50 was 23.4 mg/L.^{1 2 3}

Diethanolamine could be present in the product in concentration between 2 -5 %.

A concern for the environmental compartment has been proposed in the Registration dossier of diethanolamine, but various publications on this substance are reported on the fish endpoint, and it is not possible taken into account a key value.

All the LC50s reported on fish can be summarised to the value > 1000 mg/L.

Also on invertebrates various publications on diethanolamine are reported, and the LC50 values are in the wide range of 30 -171 mg/L.

For algae results of toxicity are in a wide range, between 2.2- 87 mg/L as well.⁴

Regarding microorganism, DIN 38412, part 8 (Pseudomonas Zellvermehrungshemm-Test) was performed on Coconut fatty acid diethanolamide. Pseudomonas putida was exposed to the similar substance and the EC50 of 60000 mg/L (inhibition of cell growth) was observed.^{1 2 3}

Long-term data

Fish

A study conducted according to OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study) and OECD Guideline 215 (Fish, Juvenile Growth Test) was performed on Amides, C12-18 (even-numbered) and C18 (unsatd.), N,N-bis(hydroxyethyl), CAS 90622-74-5.

The NOEC and LOEC for mortality, symptoms of intoxication and effects on growth were established after 28 days at nominal concentrations of 0.32 and 1.0 mg/L (i.e., equivalent to a measured concentration of 0.26 and 0.83 mg/L), respectively, in rainbow trout.^{2 3}

Aquatic invertebrates

Another long-term study was conducted according to OECD Guideline 202, Part II, Draft 6/5/91: "Prolonged Toxicity Study with Daphnia magna: Effects on Reproduction" Which is updated as OECD Guideline 211 (Daphnia magna Reproduction Test).

Under the conditions of the study, the 21 d NOEC and LOEC of amides, C12-18 (even-numbered) and C18-unsatd., N,N-bis(hydroxyethyl), CAS 90622-74 -5 were 0.07 and 0.24 mg/L (measured), respectively(based on the arithmetical mean measured concentrations of the nominal concentration of 0.1 and 0.32 mg/L) respectively.²

The same similar substance was tested twice, according to the OECD Guideline 201 on in a Scenedesmus subspicatus (new name: Desmodesmus subspicatus), for 72 hours. Under the test conditions, the relative NOECs were 2.0 mg/L and 0.3 mg/L.^{2 3}

A self-classification as harmful to aquatic life with long lasting effects has been proposed on diethanolamine, according to results on aquatic organisms tested. Daphnia magna has been tested with the EEC-guideline XI/681/86, in a semi-static condition for 21 days. The NOEC on reproduction is 0.78 mg/L, the LOEC 1.56 mg/L.⁴

References:

¹ECHA Registration Dossier Amides, C8-18 (even numbered) and C18-unsatd., N,N-bis(hydroxyethyl), CAS 68155-07-7;

²ECHA Registration Dossier Amides, C12-18 (even-numbered) and C18 (unsatd.), N,N-bis(hydroxyethyl), CAS 90622-74-5;

³ECHA Registration Dossier, Amides, C16-18 and C18-unsatd., N,N-bis(hydroxyethyl), CAS 68603-38-3;

⁴ECHA Registration Dossier 2,2'-iminodiethanol CAS 111-42 -2; SIDS Initial Assessment Report For SIAM 24 - Paris, France 17-20 April 2007 OECD: 2,2'-Iminodiethanol (CAS 111-42-2) (draft).