Propylidynetrimethanol, propoxylated, reaction products with ammonia

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 239930
- Analytical purity: 93.9 g/100 g; w(C6H11) = 25.4 g/100 g; w(C3H60) = 68.5 g/100 g

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at room temperature

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Medium: 8 ml synthetic medium 50 ml inoculum, filled up with dionised water to a total volume of 250 ml.

Test organisms (species):

activated sludge, domestic

Details on inoculum:

Activated sludge from laboratory wastewater plant treating municipal sewage.
- Initial biomass concentration: dry substance: 1 g/l

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Test temperature:

20 ± 2 °C

pH:

before correction: 6.7 - 6.8
after correction: 7.2 - 7.7

Nominal and measured concentrations:

Nominal test concentrations (mg/l): 10, 100, 248, 496, 1000

Details on test conditions:

Type: aquatic
TEST SYSTEM
- Test vessel: Erlenmeyer vessel (250 ml)
- Fill volume: 250 ml
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: 8 ml/vessel 1 00-fold concentrated OECD

OTHER TEST CONDITIONS
- Adjustment of pH: yes

Reference substance (positive control):

yes

Remarks:

3,5 dichlorphenole

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

ca. 130 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Duration:

30 min

Dose descriptor:

other: EC80

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

ca. 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

Since TS 5 (10 mg/l) delivered no feasible value, if was not considered to determine the EC20 value.

Test item	respiration	% of blank
[mg/L]	[mg O2/g TS]
Blank	20	-
10	15	75
100	17	85
248	13	65
496	13	65
1000	10	50

Conclusions:

No inhibition was observed at nominal concentrations up to 1000 mg/L. The EC50 (30 min) value was determined to be ca. 1000 mg/L for activated sewage sludge.

Description of key information

EC50 (30 min) = ca. 1000 mg/L for activated sewage sludge (OECD Guideline 209)

Key value for chemical safety assessment

EC50 for microorganisms:

1 000 mg/L

Additional information

The Klimisch 1 study by BASF AG (2003) performed a test at concentrations of 10, 100, 248, 496 and 1000 mg/L. Activated sewage sludge from laboratory wastewater plant treating municipal sewage was used in the study. The rate of respiration was determined after 30 minutes contact time. No inhibition was observed at nominal concentrations up to 1000 mg/L.