Distilled acetalization product between glucose and C12, C14, C18, C20 ,C22 alcohol

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: This study was conduced following a accepted OECD guidance and in compliance with the Good Laboratory Practice. No amendement or deviation which could have affect the quality of the study occured.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

yes

Remarks:

No analytical determination was made.

GLP compliance:

yes (incl. QA statement)

Remarks:

2007-01-12

Test material

Test material information

Constituent 1

Reference substance name:

Montanov L

IUPAC Name:

Montanov L

Details on test material:

Batch: Montanov L
Appearence: pearls of wax
Purity: ES>99%
Stability: water 96h
Solubility: water <0.1g/L
Production date: 06-09-08
Expiry date: 08-03-09
Storage: in aclosed vessel, at room temperature 20 +/- 5°C
Hazard information: none

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

As the test item is poorly soluble in water, the water accomodated fraction was tested. This was done by shaking the nominal load with the appropriate amount of deionised water for 24 hours, followed by membrane filtration.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

Unicellular fresh alga
Genus, Species: Desmodesmus subspicatus
Strain Chodat
Family: Chlorophyceae
Order: Chlorococcales
The culture of D. subspicatus was obtained in October 2006 from the "Collection of alga", Institut für Pflanzenphysiologie of Universität Göttingen. The alga are kepts as stock culture on solid agar at 8°C. From an aliquot of this stock culture, the pre-culture was prepared.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Test temperature:

23-25°C

pH:

8.1-10.1

Nominal and measured concentrations:

0 / 1 / 10 / 100 mg/L

Details on test conditions:

Vessels: round cuvette d=6mm
duration: 72 hours
Lighting: 4440-8880 LUX
Control: deionised water with nutrient medium and algae
Treatments: test solution with nutrient memdium and algae

As the test item is poorly soluble in water, the water accomodated fraction was tested. This was done by shaking the nominal load with the appropriate amount of deionised water for 24 hours, followed by membrane filtration.

Date: November, 21st - 24th 2006
Treatments tested: 1 / 10 / 100 mg/L
Number of replicates: six replicates for the control
three replicates for each treatment

In the main study, three concentrations of the test item ina geometric series (spaced by factor 10, the highest being 100 mg/L) were used. The treatments were used to incubate the unicellular freshwater green alga desmodesmus subspicatus for a period of 72 hours.
The cell concentration of each replicate was determined by measuring the absorption of the cuvettes at 440 nm every 24 hours with a spectral photometer. With these measured values, the number of cells was calculated (linear correlation between cell concentration and absorption given). Then the growth rate, the area under the curve (AUC) and the yield could be determined.

Reference substance (positive control):

yes

Remarks:

K2Cr2O7 (CAS n° 7778-50-9)

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Effect concentrations 3

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 4

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 5

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Effect concentrations 6

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 7

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 8

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Effect concentrations 9

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Results with reference substance (positive control):

Concentration tested: 0 / 0.1 / 0.16 / 0.25 / 0.4 / 0.63 / 1.0 / 1.6 mg/L
NOEC 72h 0.1 mg/L
LOEC 72h 0.16 mg/L
72h ErC50 1.1 mg/L
72h EbC50 0.36 mg/L
72h EyC50 0.30 mg/L
72h ErC100 >1.6 mg/L
72h EbC100 >1.6 mg/L
72h EyC100 > 1.6 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Duration Endpoint Effect conc. Nominal/Measured Conc. based on
72 h NOEC ca. 100 mg/L nominal test mat. growth rate
72 h NOEC ca. 100 mg/L nominal test mat. cell number
72 h NOEC ca. 100 mg/L nominal test mat. biomass
72 h LOEC > 100 mg/L nominal test mat. growth rate
72 h LOEC > 100 mg/L nominal test mat. cell number
72 h LOEC > 100 mg/L nominal test mat. biomass
72 h EC50 > 100 mg/L nominal test mat. growth rate
72 h EC50 > 100 mg/L nominal test mat. cell number
72 h EC50 > 100 mg/L nominal test mat. biomass

Executive summary:

Determination of 72h-EC50 of Montanov L against Desmodesmus subspicatus according to OECD 201 resp. EU C.3.

This study was performed in order to evaluate the toxic potential of Montanov L towards Desmodesmus subspicatus CHODAT, an unicellular freshwater alga. The incubation period of 72h corresponds to a subchronic test, as during this period, about five cell divisions take place. Selection of the test system was made following the proposal of the guidelines.

The study was conduced in accordance with the following guidelines:

OECD guideline for the testing of chemicals n° 201 "alga growth inhibition test"

EU-guideline C.3 "alga inhibition test"

As the test item is poorly soluble in water, the water accomodated fraction was tested. This was done by shaking the nominal load with the appropriate amount of deionised water for 24 hours, followed by membrane filtration.

In the main study, three concentrations of the test item ina geometric series (spaced by factor 10, the highest being 100 mg/L) were used. The treatments were used to incubate the unicellular freshwater green alga desmodesmus subspicatus for a period of 72 hours.

The cell concentration of each replicate was determined by measuring the absorption of the cuvettes at 440 nm every 24 hours with a spectral photometer. With these measured values, the number of cells was calculated (linear correlation between cell concentration and absorption given). Then the growth rate, the area under the curve (AUC) and the yield could be determined.

The EC50 of potassium dichromate was tested in a curent reference test. The values lay within the normal range of the laboratory.

The following results for Montanov L were determined.

72 h NOEC (growth rate) = 100 mg/L

72 hNOEC (AUC) = 100 mg/L

72 hNOEC (yield) = 100 mg/L

72 hLOEC (growth rate) > 100 mg/L

72 hLOEC (AUC) > 100 mg/L

72 hLOEC (yield) > 100 mg/L

72 hEC50 (growth rate) > 100 mg/L

72 hEC50 (AUC) > 100 mg/L

72 hEC50 (yield) > 100 mg/L