2-methylimidazole

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1966

Report date:

1966

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Principles of method if other than guideline:

BASF test. 50 µl of the test substance were applied to the conjunctival sac of one eye in 2 animals. The animals were observed after 10 min, 1 and 3 h on the day of treatment and up to 8 days afterwards. Findings were recorded daily. The eyes were not washed out after 24 hours as specified in OECD Guideline 405. The report describes findings after 1 and 24 hours and at the end of the observation period.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-methylimidazole

EC Number:

211-765-7

EC Name:

2-methylimidazole

Cas Number:

693-98-1

Molecular formula:

C4H6N2

IUPAC Name:

2-methyl-1H-imidazole

Details on test material:

- Name of test material (as cited in study report): 2-Methylimidazol

Test animals / tissue source

Species:

rabbit

Strain:

Vienna White

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: The adjacent eye served as control with talc powder.

Amount / concentration applied:

TEST MATERIAL
- Amount applied: 50 µl

Duration of treatment / exposure:

single application, no washing was performed

Observation period (in vivo):

8 days

Number of animals or in vitro replicates:

2

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing (if done): no

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

chemosis score

Basis:

mean

Time point:

other: 1 h

Score:

4

Max. score:

4

Reversibility:

fully reversible within: 24 h

Remarks on result:

other: Cauterization of mucous membrane was observed from observation time period 1 hour and this was not reversible.

Results 2

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

other: 1 h - 24 h - 8d

Score:

3

Max. score:

4

Reversibility:

not reversible

Remarks on result:

other: Cauterization of mucous membrane was observed from observation time period 1 hour and this was not reversible.

Results 3

Irritation parameter:

conjunctivae score

Basis:

mean

Time point:

other: 1 h - 24 h

Score:

3

Max. score:

3

Reversibility:

fully reversible within: 8 d

Remarks on result:

other: Cauterization of mucous membrane was observed from observation time period 1 hour and this was not reversible.

Irritant / corrosive response data:

Cauterization of mucous membrane was observed from observation time period 1 hour and this was not reversible.