N-[3-(dimethylamino)propyl]-N,N',N'-trimethylpropane-1,3-diamine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

June 16, 1997 - June 20, 1997

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

See remarks under Principle of method if other than guideline.

Principles of method if other than guideline:

Deviation:
The average zebra fish length at the study initiation 3.4 cm (range 2.7 - 3.7 cm) is higher than the OECD 203 test guideline recommendation for the zebra fish (2.0 ± 1.0 cm). No explaination was provided for this deviation.
However, fish were physically examined before use to ensure no abnormalities.
Weight: 0 .26 (0 .16 - 0 .38) g
Length: 3 .4 (2 .7 - 3 .7) cm
Physical conclitions : no abnormalities

Otherwise, constant conditionswere maintained hroughout the test, meeting the validity criteria laid out in OECD TG 203:
- Test system temperature was maintained at 22-23C throughout the test.
- Dissolved oxygen concentrations were lower at 96hrs, however these remained above 60% of maximum saturation throughout the test (range 5.7mg/L - 8.2)
- Analytically detected concentrations after 1h and 96h were in the range 88-13% of the niminal concentrations.
- pH values remained in the range 8.2-9.2 throughout.

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Name of test subst:ance : N ;N ;N' ;N " ;N " -Pentamethyldipropylentriamin
Substance number : 96/10 7
CAS-No . :3855-32-1
Degree of purity: 98 .5 %(see analytical report ZAX 96L00390 from Sept . 1996 )
Date of production: April 1995
Physical state/appearance : Liquid, colorles s
Storage conditions : Room temperature, under nitrogen atmosphere
Stability : The stability of the test substance over the study period was proved by reanalysis .
Solubility in water : Soluble up to 10 g/1

Analytical monitoring:

yes

Details on sampling:

The analytically detected concentrations were initially in the range of 97.0% - 102 .3% and after about 96 hours between 88 .O% - 90 .7% of the nominal concentrations. See attached results.

Vehicle:

no

Details on test solutions:

Separately for each concentration 0 .1006, 0 .2154, 0 .4648, 1 .00, 2 .15, 4 .64 and 4 .64 (pH-adjustment) g test substance were added to the test water using an ultra-turrax-stirrer .
From the beginning of preparing the test solutions until termination of placing the fish into the aquaria 20 minutes were needed .

Municipal water of the city of Frankenthal, not chlorinated and passed through a charcoal filter, and aerated, was used.
Total hardness : about 2 .5 mmol/ 1
(= about 250 mg/1 CaC03)
Acid capacity : about 5 .5 mmol/ 1
Ca content : about 90 mg/1
Mg content : about 10 mg/1
pH : about 8 .0 - 8 . 6
The test water is regularly assayed for chemical contaminants by the municipal authorities of Frankenthal and the technical services of BASF Aktiengesellschaft as well as for presence of microbes by a contract laboratory .

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
- Source: Charles River Aquatics, Someren, Netherlands
- Age at study initiation (mean and range, SD): about 4months
- Length at study initiation (length definition, mean and range): 3.4 (2.7 - 3.7) cm
- Weight at study initiation (mean and range): 0.25 (0.16 - 0.38) g
- Feeding during test: withdrawal of food one day before and during exposure

ACCLIMATION
- Acclimation period: about one month
- Acclimation conditions (same as test or not): yes
- Type and amount of food:TETRA MIN standard feed for aquarium fish; on workdays generally additionally live artemia
- Feeding frequency: ad libidum
- Health during acclimation (any mortality observed): no medical treatment; no abnormalities at start of test.

Animal breeder/supplier : Charles River Aquatics, Someren ; Netherlands

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

Total hardness in test water: about 2.5 mmol/L (= about 250 mg/1 CaC03)

Test temperature:

22 - 23 degrees C (temperature-controlled room)

pH:

about 8 .0 - 8 . 6

Dissolved oxygen:

> 60% of maximum saturation (see attached results)

Salinity:

NA

Conductivity:

NA

Nominal and measured concentrations:

Test concentrations: 0 mg/L (nominal); 10.0 mg/L (nominal); 21.5 mg/L (nominal); 46.4 mg/L (nominal); 100.0 mg/L (nominal); 215 mg/L (nominal); 464 mg/L (nominal); 464 mg/L (pH-adjusted, nominal).

Details on test conditions:

Aeration of the test system: None
Photoperiod: 16 hours light and 8 hours darkness
Volume of water: 10 liters (water depth about 17 cm)
No . of animals per vessel : 10
Loading (g fish / 1 water) : 0.3 g/L
Test temperature : 22 - 23 degrees Celsius (temperature-controlled room )
Adaptation: Test conditions corresponded to housing conditions .
Withdrawal of food: 1 day before and during exposure

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 92.5 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

>= 20.5 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

>= 206.5 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 21.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

>= 215 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

To evaluate the possible effect of the relatively high pH, the highest test concentration (464 mg/L nominal) was tested in parallel with pH adjustment to the pH of the control. The pH adjustment did not significantly reduce mortality or toxic signs.

Validity criteria fulfilled:

yes

Remarks:

However, the average zebra fish length at the study initiation 3.4 cm (range 2.7 - 3.7 cm) is higher than the OECD 203 test guideline recommendation for the zebra fish (2.0 ± 1.0 cm). No explanation was provided for this deviation.

Conclusions:

An acute toxicity study on the effects of N;N;N';N'';N''-Pentamethyldipropylentriamin on zebra fish (Brachdanio rerio HAM. and BUCH.) was conducted. Based on nominal concentrations, the 96-h LC50 was reported as ca. 100 mg/L. Mortality occurred first at the concentration of 46.4 mg/L (nominal). Other substance-related effects were the symptoms of apathy, tumbling, and swimming near the water surface. The NOEC was reported as 21.5 mg/L (nominal). Based on the arithmetic mean values of the analytically detected concentrations after 1+96 hours, the LC50 was reported at about 92.5 mg/L. The pH adjustment of a parallel group with the highest nominal concentration (464 mg/L) did not significantly reduce mortality and toxic signs.

Executive summary:

An acute toxicity study on the effects of N;N;N';N'';N''-Pentamethyldipropylentriamin on zebra fish (Brachdanio rerio HAM. and BUCH.) was conducted according to OECD TG 208 (Fish, Acute Toxicity Test).

Aquaria containing 10 liter were loaded with 10 fish each. The fish were allocated to the aquaria following a randomization plan of the laboratory.

A static procedure was used.

Test concentrations were selected on the basis of 2 preliminary studies conducted at the BASF Aktiengesellschaft, Department of Toxicology .

Results of the 2 range findiing studies (LC 50 after 96 h : between 100 and 1000 mg/1) the concentrations, spaced by a factor of about 2 .2 , were fixed as follows

0, 10 .0, 21 .5, 46 .4, 100 .0, 215, 464, 464 (pH adjusted) mg/L.

There was no mortality reported in the controls, therefore the validity criterion was reached (<10 mortality).

Although a static test was performed, constant conditionswere maintained  throughout the test:

- Test system temperature was maintained at 22-23C throughout the test.

- Dissolved oxygen concentrations were lower at 96hrs, however these remained above 60% of maximum saturation throughout the test (range 5.7mg/L - 8.2)

- Analytically detected concentrations after 1h and 96h were in the range 88-13% of teh niminal concentrations.

Details are provided in the attached results tables.

The average zebra fish length at the study initiation 3.4 cm (range 2.7 - 3.7 cm) is higher than the OECD 203 test guideline recommendation for the zebra fish (2.0 ± 1.0 cm). No explaination was provided for this deviation.

However, fish were physically examined before use to ensure no abnormalities.

Weight: 0 .26 (0 .16 - 0 .38) g

Length: 3 .4 (2 .7 - 3 .7) cm

Physical conclitions : no abnormalitie s

The death of 1 fish at the lowest concentration (10 .0 mg/1) was obviously not substance-related, and since it was not concentration-related, it was theref'ore not considered for the results .

The pH-value of a parallel group of the highest concentrat.ion (= 464 mg/1) was adjusted with 32x HC1 ta approximately the pH of the control .

Based on nominal concentrations, the 96-h LC50 was reported as ca. 100 mg/L. Mortality occurred first at the concentration of 46.4 mg/L (nominal).  Other substance-related effects were the symptoms of apathy, tumbling, and swimming near the water surface.  The NOEC was reported as 21.5 mg/L (nominal).  Based on the arithmetic mean values of the analytically detected concentrations after 1+96 hours, the LC50 was reported at about 92.5 mg/L.  The pH adjustment of a parallel group with the highest nominal concentration (464 mg/L) did not significantly reduce mortality and toxic signs.

Description of key information

An acute toxicity study on the effects of the substance on zebra fish (Brachydanio rerio HAM. and BUCH.) was conducted in accordance with OECD 203 (April 1984, updated July 1992). 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

92.5 mg/L

Additional information

An acute toxicity study on the effects of the substance on zebra fish (Brachydanio rerio HAM. and BUCH.) was conducted. Based on measured concentrations, the 96-h LC50 was reported as 92.5 mg/L. Mortality occurred first at the concentration of 46.4 mg/L (nominal). Other substance-related effects were the symptoms of apathy, tumbling, and swimming near the water surface. The NOEC was reported as 21.5 mg/L (nominal). Based on the arithmetic mean values of the analytically detected concentrations after 96 hours, the LC50 was reported at about 92.5 mg/L. The pH adjustment of a parallel group with the highest nominal concentration (464 mg/L) did not significantly reduce mortality and toxic signs.