Reaction mass of Dimethyl [3-[(hydroxymethyl) amino]-3-oxopropyl] phosphonate and Dimethyl (3-amino-3-oxopropyl)phosphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992-01-16 - 1992-07-09

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Specific details on test material used for the study:

Test substance FAT-80'001/I
Stability: guaranteed by the sponsor
Solubility: >50 g/l (in water)
Storage: room temperature
Appearance: liquid

Analytical monitoring:

yes

Details on sampling:

ANALYTICAL CONTROL
Water samples were taken from the bath at the beginning and at the end of the experiment.

Vehicle:

no

Details on test solutions:

Diluted In dechlorinated tap water (carbon filter)

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Test organism: Zebrafish (Brachidanio rerio)
Weight: 2.0 - 2.3 g
Length: 28 - 29 mm
Age: 83 days
Acclimatization: 12 days in dechlorinated tap water
Supplier: West Aquarium, Bad Lauterberg, BRD

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

179 mg/l CaCO3

Test temperature:

actual 21.9 - 22.9 °C ± 0.5 °C (nominal 21-25 °C within ± 1°C)

pH:

7.7 - 8.3

Dissolved oxygen:

79 - 85 %

Nominal and measured concentrations:

A pretest was carried out at 30,100, 300 and 1000 mg/l.
For the main test the concentrations were selected according to the results of the pretest as: 316, 562 and 1000 mg/l (nominal).

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

978 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortality was seen throughout the study.
No abnormal responses of the test fish were observed.
The measured concentrations were in the range of 89.2-100.2 % of the nominal concentrations.

Validity criteria fulfilled:

yes

Conclusions:

The test item had no acute toxic effects on zebra fish under the present test conditions at a nominal concentration of 1000 mg/L.

Executive summary:

DMPPA_701-402-5 was assessed for toxicity to fish in a 96 hour study conducted according to OECD Guideline 203. Based on the results of the pre-study, 4 groups of 10 Zebra fish each were exposed to test concentrations of 316, 562 and 1000 mg/L (nominal). No mortality was seen throughout the study and no abnormal responses of the test fish were observed. The measured concentrations were in the range of 89.2-100.2 % of the nominal concentrations. Based on the findings of the study, the LC50 was determined to be >1000 mg/L.

Description of key information

The acute aquatic toxicity of the test item was tested in a valid GLP study in accordance with EU Method C.1 by exposing zebra fish (Danio rerio) during 96 hours in a static test to a nominal concentration of 316, 562 and 1000 mg/L. No adverse effects were observed and the resulting LC50 value was >1000 mg/L. The above outcome was further supported by the finding of 48h LC50 to be 2750 mg/L in a supporting study conducted according to DIN 38412, Teil 15.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 000 mg/L