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EC number: 238-475-3 | CAS number: 14481-26-6
Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2019-05-21 to 2019-10-17
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental study performed according to the OECD 221 guideline. The validity criteria are met.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 221 (Lemna sp. Growth Inhibition Test)
- Version / remarks:
- 2006
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Lemna minor
- Details on test organisms:
- The starter culture of Lemna minor was provided by ECT Oekotoxikologie.
Seven days before testing, sufficient colonies of the laboratory stock culture of Lemna minor were transferred aseptically into fresh sterile Steinberg medium and cultured under the conditions of the test.
Culture conditions are the following:
Medium: Steinberg medium
Temperature: 24°C ± 2
Light: continuous illumination between 6 500 and 10 000 lux - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 7 d
- Nominal and measured concentrations:
- Nominal: 30; 40.6; 54.8; 74 and 100 mg test item. L-1
Measured: 18.1; 23.1; 28.9; 39.7 and 57.9 mg test item.L-1 (geo mean) - Key result
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 91.5 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- frond number
- Key result
- Duration:
- 7 d
- Dose descriptor:
- EC10
- Effect conc.:
- 39.6 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- frond number
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 93 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: dry weight parameter
- Duration:
- 7 d
- Dose descriptor:
- EC10
- Effect conc.:
- 53.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: dry weight parameter
- Validity criteria fulfilled:
- yes
- Conclusions:
- Please, see the executive summary below
- Executive summary:
The test item is a soluble substance under the test conditions and the test solutions were prepared by dilution of a primary stock solution and by directly dissolving the test item in the test solution.The effect of Titanium and potassium oxalate diluted in OECD LD 221 test medium at the definitive rates of 30; 40.6; 54.8; 74 and 100 mg test item. L-1onLemna minorwas investigated under laboratory and semi statics conditions.
Test item quantification was performed according to a validated analytical method (final report RRCo-000452_01) using a ICS-5000+ from Thermo (Dionex) ion chromatography to measure Titanium and potassium oxalate concentrations at 0-day and 7-day and at 2,3,4,5,6-day before and after renewal.
Since the deviation between the analysed concentrations before and after renewal was not always within the range of ± 20 % and since the test has been performed in semi-static conditions, analysis of the results were based on the geometrical mean exposure concentrations (18.1; 23.1; 28.9; 39.7 and 57.9 mg test item.L-1). A precipitate was occurred during the exposition probably due to a lower test item solubility in the test media.
The study is valid since average specific growth rate in the control is > 0.275 per day. (actual value: 0.3637).
All ECx, based on geometrical mean concentrations are summarized in the table below.
Titanium and potassium oxalate
Frond number parameter
ErC10(95% confidence limits)
39.6mg.L-1(28.3–53.4mg.L-1)
ErC50(95% confidence limits)
91.5mg.L-1(65.1–160.6mg.L-1)
EyC10(95% confidence limits)
26.9mg.L-1(11.0–46.6mg.L-1)
EyC50(95% confidence limits)
59.1mg.L-1(48.8–91.1mg.L-1)
Dry weight parameter
ErC10(95% confidence limits)
53.1mg.L-1(40.6–62.4mg.L-1)
ErC50(95% confidence limits)
93.0mg.L-1(64.0–258.0mg.L-1)
EyC10(95% confidence limits)
40.3mg.L-1(20.7–55.6mg.L-1)
EyC50(95% confidence limits)
69.2mg.L-1(58.1–122.5mg.L-1)
Based on the average specific growth or yield for frond number, the NOEC was 39.7 mg test item.L-1and the LOEC was 57.9 mg test item.L-1.
Based on dry weights parameters, the NOEC was 57.9 mg test item.L-1and the LOEC was estimated to be>57.9 mg test item.L-1.
Reference
Description of key information
Experimental study performed according to OECD 221 guideline and under GLP conditions. The validity criteria are met and the study is therefore considered acceptable for that endpoint.
Key value for chemical safety assessment
- EC50 for freshwater plants:
- 91.5 mg/L
- EC10 or NOEC for freshwater plants:
- 39.6 mg/L
Additional information
The test item is a soluble substance under the test conditions and the test solutions were prepared by dilution of a primary stock solution and by directly dissolving the test item in the test solution.
The effect of Titanium and potassium oxalate diluted in OECD LD 221 test medium at the definitive rates of 30; 40.6; 54.8; 74 and 100 mg test item. L-1onLemna minorwas investigated under laboratory and semi statics conditions.
Test item quantification was performed according to a validated analytical method (final report RRCo-000452_01) using a ICS-5000+ from Thermo (Dionex) ion chromatography to measure Titanium and potassium oxalate concentrations at 0-day and 7-day and at 2,3,4,5,6-day before and after renewal.
Since the deviation between the analysed concentrations before and after renewal was not always within the range of ± 20 % and since the test has been performed in semi-static conditions, analysis of the results were based on the geometrical mean exposure concentrations (18.1; 23.1; 28.9; 39.7 and 57.9 mg test item.L-1). A precipitate was occurred during the exposition probably due to a lower test item solubility in the test media.
The study is valid since average specific growth rate in the control is > 0.275 per day. (actual value: 0.3637).
All ECx, based on geometrical mean concentrations are summarized in the table below.
|
Titanium and potassium oxalate |
Frond number parameter |
|
ErC10(95% confidence limits) |
39.6mg.L-1(28.3–53.4mg.L-1) |
ErC50(95% confidence limits) |
91.5mg.L-1(65.1–160.6mg.L-1) |
EyC10(95% confidence limits) |
26.9mg.L-1(11.0–46.6mg.L-1) |
EyC50(95% confidence limits) |
59.1mg.L-1(48.8–91.1mg.L-1) |
|
|
Dry weight parameter |
|
ErC10(95% confidence limits) |
53.1mg.L-1(40.6–62.4mg.L-1) |
ErC50(95% confidence limits) |
93.0mg.L-1(64.0–258.0mg.L-1) |
EyC10(95% confidence limits) |
40.3mg.L-1(20.7–55.6mg.L-1) |
EyC50(95% confidence limits) |
69.2mg.L-1(58.1–122.5mg.L-1) |
Based on the average specific growth or yield for frond number, the NOEC was 39.7 mg test item.L-1and the LOEC was 57.9 mg test item.L-1.
Based on dry weights parameters, the NOEC was 57.9 mg test item.L-1and the LOEC was estimated to be>57.9 mg test item.L-1.
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