Pentane-2,3-dione

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

significant methodological deficiencies

Data source

Reference

Reference Type:

publication

Title:

Structural Determinants in the Oxidation of Aliphatic Compounds by Activated Sludge

Author:

Gerhold, RM and Malaney GW

Year:

1966

Bibliographic source:

Journal (Water Pollution Control Federation), 38(4): 562–579

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

- Principle of test:
500 mg/l test material and a mixed liquor of municpal activated sludge (detailed below) were tested in a flask at a total volume of 20 ml was placed in a water bath at 20°C. Oxygen uptake was determined for 24 hours, with measurements at 0.5-5 hour intervals.

Activated sludge was obtained from the following source:
1. The Columbus, Ohio, municipal treatment plant, which treated 70 mgd (265,000 m³/day) of combined do mestic and industrial wastes. Suspended solids concentrations in the plant mixed liquor averaged 4200 mg/l.
2. The Hilliard, Ohio, municipal plant, which treated 450,000 gpd (1,700 m³/day) of domestic waste water. Suspended solids averaged 1,600 mg/l.
3. The Brookside Estates treatment plant at Linworth, Ohio, which treated 37,000 gpd (140 m³/day) of residential wastes. Suspended solids averaged 2,900 mg/l.

- Parameters analysed / observed:
Oxygen uptake

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Pentane-2,3-dione

EC Number:

209-984-8

EC Name:

Pentane-2,3-dione

Cas Number:

600-14-6

Molecular formula:

C5H8O2

IUPAC Name:

pentane-2,3-dione

Test material form:

liquid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

Activated sludge was obtained from the following source:
1. The Columbus, Ohio, municipal treatment plant, which treated 70 mgd (265,000 m³/day) of combined do mestic and industrial wastes. Suspended solids concentrations in the plant mixed liquor averaged 4200 mg/l.
2. The Hilliard, Ohio, municipal plant, which treated 450,000 gpd (1,700 m³/day) of domestic waste water. Suspended solids averaged 1,600 mg/l.
3. The Brookside Estates treatment plant at Linworth, Ohio, which treated 37,000 gpd (140 m³/day) of residential wastes. Suspended solids averaged 2,900 mg/l.

Mixed liquor was obtained fom the aeration tank on the day of the experiment. Supended solids concentration was adjusted to 2500 mg/l by either removal of supernatant liquid or addition of tap water. The sludge was not washed and no mineral salts were added.

Duration of test (contact time):

24 h

Initial test substance concentration

Initial conc.:

500 mg/L

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Sampling time:

6 h

Remarks on result:

not determinable because of methodological limitations

Remarks:

Test material was reported to be 'toxic'

% Degradation 2

Parameter:

% degradation (O2 consumption)

Sampling time:

12 h

Remarks on result:

not determinable

Remarks:

Test material was reported to be 'toxic'

% Degradation 3

Parameter:

% degradation (O2 consumption)

Sampling time:

24 h

Remarks on result:

not determinable

Remarks:

Test material was reported to be 'toxic'

Applicant's summary and conclusion

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The theoretical oxygen demand of the test material could not be determined as the test concentration was toxic to microorganisms. Therefore, under the test conditions, it is assumed that no biodegradation was observed.