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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
18 Aug - 27 Aug 1969
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Remarks:
study protocol is in principle similar to OECD TG 403, limited documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1970
Report date:
1970

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Principles of method if other than guideline:
The test demonstrates the toxicity of an atmosphere saturated with vapours of the volatile components of a test substance at the temperature chosen for vapour generation (20 °C). 6 rats per sex were exposed to the vapours or aerosol, generated by bubbling 200 L air/h through a substance column of about 5 cm above a fritted glassdisc in a glass cylinder for 8 h. The documentation of clinical signs was performed over a period of 7 days.
GLP compliance:
no
Test type:
other: inhalation hazard test

Test material

Constituent 1
Chemical structure
Reference substance name:
N,N'-bis(3-aminopropyl)propane-1,3-diamine
EC Number:
225-007-8
EC Name:
N,N'-bis(3-aminopropyl)propane-1,3-diamine
Cas Number:
4605-14-5
Molecular formula:
C9H24N4
IUPAC Name:
N,N'-bis(3-aminopropyl)propane-1,3-diamine

Test animals

Species:
rat
Strain:
other: Gassner rat
Sex:
male/female

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
not specified
Vehicle:
air
Analytical verification of test atmosphere concentrations:
not specified
Duration of exposure:
8 h
Concentrations:
saturated vapor atmosphere
No. of animals per sex per dose:
6
Control animals:
no
Details on study design:
- Duration of observation period following administration: 7 days
- Other examinations performed: clinical signs
- Frequency of observations: several times on the day of application and daily thereafter
- 7 days post exposure animals were sacrificed and underwent necropsy.
- Pathologically investigated: heart, lung, liver, kidney, adrenal gland, stomach, intestine, bladder, genitals, traches, central nervous system, bone marrow, tumor, blood, urine

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
other: Inhalation Risk Test
Exp. duration:
8 h
Remarks on result:
other: no mortality occured
Mortality:
No deaths
Clinical signs:
other: violent escape attempts, mucosal irritation
Body weight:
no evaluable data
Gross pathology:
no findings

Applicant's summary and conclusion

Conclusions:
The inhalation of a highly saturated vapor-air-mixture caused no mortality but clincal signs, after 8 h of exposure.