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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
19 Aug - 27 Aug 1969
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Remarks:
study protocol is in principle similar to OECD TG 401, limited documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1970
Report date:
1970

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Principles of method if other than guideline:
BASF-Test: Animals were treated by single gavage application with an aqueous solution of the test substance. The animals were observed for mortality and for clinical symptoms of toxicity. At the end of the observation period of 7 days, the surviving animals were sacrificed for the purpose of necropsy; animals that died during the observations period also were subjected to necropsy.
GLP compliance:
no
Test type:
other: BASF-Test
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
N,N'-bis(3-aminopropyl)propane-1,3-diamine
EC Number:
225-007-8
EC Name:
N,N'-bis(3-aminopropyl)propane-1,3-diamine
Cas Number:
4605-14-5
Molecular formula:
C9H24N4
IUPAC Name:
N,N'-bis(3-aminopropyl)propane-1,3-diamine

Test animals

Species:
rat
Strain:
other: Gassner rat
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Willi Gassner, Sulzfeld, Germany
- Age at study initiation: young adults
- Weight at study initiation: 142 - 188 g

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
water
Remarks:
aqueous emulsion with Traganth
Details on oral exposure:
VEHICLE
- Concentration in vehicle: 20%, 10%, 8%, 4%, 2% aqueous emulsion with Traganth
Doses:
200, 400, 500, 640, 800, 1000, 1250, 1600 mm3/kg bw (corresponding to 184, 386, 460, 588.8, 736, 920, 1150, 1472 mg/kg bw; calculated using the density of 0.92 g/cm³)
No. of animals per sex per dose:
10
Control animals:
no
Details on study design:
- Duration of observation period following administration: 7 days
- Other examinations performed: clinical signs
- Frequency of observations: several times on the day of application and daily thereafter
- Pathologically investigated: heart, lung, liver, kidney, adrenal gland, stomach, intestine, bladder, genitals, trachea, central nervous system, bone marrow, tumor, blood, urine

Results and discussion

Effect levels
Key result
Sex:
male/female
Dose descriptor:
LD50
Effect level:
736 mg/kg bw
Mortality:
1472 mg/kg 10/10 m, 10/10 w
1150 mg/kg 4/10 m, 10/10 w
920 mg/kg 4/10 m, 5/10 w
736 mg/kg 1/10 m, 9/10 w
588.8 mg/kg 0/10 m, 0/10 w
460 mg/kg 0/10 m, 2/10 w
386 mg/kg 0/10 m, 0/10 w
184 mg/kg 0/10 m, 0/10 w
Clinical signs:
1472 mg/kg bw: No symptoms on the first day of the trial, the following day rough fur, irregular breathing, apathy. Within 24 hours post applicaion, all animals died
1150 - 460 mg/kg : Huddled together about 10 minutes after application, quiet restrained, shock or irregular breathing and semi-closed eyes. The first six animals died within the first 24 hours,
386 - 184 mg/kg: No symptoms on the first day of the trial, but on the second day rough fur and irregular breathing.
Body weight:
no data
Gross pathology:
Sacrificed animals: Frequently strongly dilated stomachs and sagging intestinal tract. Rot.

Applicant's summary and conclusion

Interpretation of results:
Category 4 based on GHS criteria