Tall oil, polymd., oxidized

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2009-04-24 to 2009-04-29

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Concentrations: 100 mg/L

Vehicle:

no

Test organisms (species):

activated sludge

Details on inoculum:

- Pretreatment: The activated sludge was washed three times with tap water and filtered using paper filter.
- Initial biomass concentration: The recommended amount of wet sludge for test vessels was calculated to obtain a suspended solids level of 4 g/L (±10%)

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

3 h

Test temperature:

19.9 - 20.9 °C

Dissolved oxygen:

The preparations (control 1, 1 test loading rate of the test item, reference item in 3 test concentrations, control 2) were made up in 15 minute intervals and were aerated continuously so that a dissolved oxygen level of ≥ 6.5 mg/L was achieved during the whole test duration.

Details on test conditions:

TEST SYSTEM
- Test vessel: Control 1, 1 test loading rate of test item, reference item in 3 test concentrations, control 2.
- Type (delete if not applicable): open
- Aeration: Continuous, membrane pump
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- Biomass loading rate: 100 mg/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Deionised water

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 100 mg/L

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Duration:

3 h

Dose descriptor:

other: EL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: respiration rate

Remarks on result:

other: 95% CL, Significant level 0.05

Results with reference substance (positive control):

Deviation between respiration rates of the controls, 14.5%
Relevant effect levels: EC50 (3 h): 12.2 mg/L, NOEC < 5.0 mg/L

Reported statistics and error estimates:

Determination of the EC50 of the reference item 3,5-dichlorophenol was accomplished using Probit-analysis (Finney-method, lognormal distribution, confidence limit 95%, significance level: 0.05).

Table2:

Results of the Activated Sludge, Respiration Inhibition Test of CTO Sample 5

	c / Loading rate (mg/L)	Respiration rate (mg O2/L x h)	Inhibition (%)
Control 1	-	53.3	-
Reference	3	47.1	18.6
Reference	10	36.4	37.1
Reference	30	12.8	77.9
Test item	100	70.6	-22.1*
Control 2	-	62.3	-

Remark: * The negative inhibition value indicates, that the respiration rate was higher compared to the control.

 

Validity criteria fulfilled:

yes

Conclusions:

A Water Accommodated Fractions (WAFs) value EL50 of > 100 mg/L and a NOELR of 100 mg/L have been determined for CTO in a test conducted according to OECD 209. The result is considered reliable.

Reference 2

Endpoint:

activated sludge respiration inhibition testing

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Justification for type of information:

See attached read-across justification report

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Duration:

3 h

Dose descriptor:

other: EL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: respiration rate

Remarks on result:

other: 95% CL, Significant level 0.05

Conclusions:

A Water Accommodated Fractions (WAFs) value EL50 of > 100 mg/L and a NOELR of 100 mg/L have been determined for Tall Oil, Polymd. oxidized, based on
the result of the source study (see read across justification report).

Reference 3

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2009-04-24 to 2009-04-29

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Concentrations: Different laoding rates (5 - 100 mg/l)

Vehicle:

no

Test organisms (species):

activated sludge

Details on inoculum:

Laboratory culture:
- Pretreatment: The activated sludge was washed three times with tap water and filtered using paper filter
- Initial biomass concentration: The recommended amount of wet sludge for test vessels was calculated to obtain a suspended solids level of 4 g/L (±10%)

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

3 h

Test temperature:

19.9 - 20.9 °C

Dissolved oxygen:

The preparations (control 1, 1 test loading rate of the test item, reference item in 3 test concentrations, control 2) were made up in 15 minute intervals and were aerated continuously so that a dissolved oxygen level of ≥ 6.5 mg/L was achieved during the whole test duration.

Details on test conditions:

TEST SYSTEM
- Test vessel: Control 1, 1 test loading rate of test item, reference item in 3 test concentrations, control 2.
- Type (delete if not applicable): open
- Aeration: Continuous, membrane pump
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- Biomass loading rate: 100 mg/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Deionised water

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 100 mg/L

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Duration:

3 h

Dose descriptor:

other: EL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: respiration rate

Remarks on result:

other: 95% CL, Significant level 0.05

Results with reference substance (positive control):

Deviation between respiration rates of the controls, 14.5%
Relevant effect levels: Yes: Deviation between respiration rates of the controls, 14.5%

Reported statistics and error estimates:

Determination of the EC50 of the reference item 3,5-dichlorophenol was accomplished using Probit-analysis (Finney-method, lognormal distribution, confidence limit 95%, significance level: 0.05).

Table2:

Results of the Activated Sludge, Respiration Inhibition Test of CTO Sample 3

	c / Loading rate (mg/L)	Respiration rate (mg O2/L x h)	Inhibition (%)
Control 1	-	53.3	-
Reference	3	47.1	18.6
Reference	10	36.4	37.1
Reference	30	12.8	77.9
Test item	5	55.8	3.5
Test item	10	64.9	-12.2*
Test item	25	75.0	-29.7*
Test item	50	69.6	-20.3*
Test item	100	70.6	-22.1*
Control 2	-	62.3	-

Remark: * Negative inhibition values indicate, that respiration rates were higher compared to the control. Taking into account the variation of results in the control and at the test loading rates of CTO Sample 3 the result of inhibition of 3.5 % at test loading rate of 5 mg/L is not regarded as a definite inhibition.

 

Validity criteria fulfilled:

yes

Conclusions:

A Water Accommodated Fractions (WAFs) value EL50 of > 100 mg/L and a NOELR of 100 mg/L have been determined for CTO in a test conducted according to OECD 209. The result is considered reliable.

Description of key information

Water Accommodated Fractions (WAFs) of two CTO Samples caused no acute toxic effects on bacteria in an Activated Sludge, Respiration Inhibition Test as Limit Test conducted according to OECD 209. A 3 h EL50 of > 100 mg/L and a NOELR of 100 mg/L have been determined.

Based on the result of the source studies, a Water Accommodated Fractions (WAFs) value EL50 of > 100 mg/L and a NOELR of 100 mg/L have been determined for Tall Oil, Polymd. oxidized (see read across justification report).

Key value for chemical safety assessment

EC50 for microorganisms:

100 mg/L

EC10 or NOEC for microorganisms:

100 mg/L