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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
not specified
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
abstract

Data source

Reference
Reference Type:
publication
Title:
Toxicology Update Triethylene glycol HO(CH2CH2O)3H
Author:
Bryan Ballantyne, William M. Snellings
Year:
2007
Bibliographic source:
J. Appl. Toxicol. 2007; 27: 291–299 Published online 14 February 2007 in Wiley InterScience

Materials and methods

Test guideline
Qualifier:
no guideline available
Version / remarks:
guideline not specified in the publication
Principles of method if other than guideline:
Salmonella typhimurium reverse mutation assay with and without metabolic activation
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
2,2'-(ethylenedioxy)diethanol
EC Number:
203-953-2
EC Name:
2,2'-(ethylenedioxy)diethanol
Cas Number:
112-27-6
Molecular formula:
C6H14O4
IUPAC Name:
2,2'-[ethane-1,2-diylbis(oxy)]diethanol

Method

Species / strain
Species / strain / cell type:
S. typhimurium, other: TA 98, TA 100, TA 1535, TA 1537, TA 1538
Metabolic activation:
with and without
Test concentrations with justification for top dose:
1-113 mg/plate
Vehicle / solvent:
not specified
Controls
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
not specified
True negative controls:
not specified
Positive controls:
not specified
Remarks:
no details given in the publication
Details on test system and experimental conditions:
no further details

Results and discussion

Test results
Key result
Species / strain:
S. typhimurium, other: TA 98, TA 100, TA 1535, TA 1537, TA 1538
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Additional information on results:
There was no evidence for an increased mutation frequency either in the presence or absence of metabolic activation.

Applicant's summary and conclusion

Conclusions:
Several in vitro genotoxicity studies have been conducted which showed that TEG is devoid of a mutagenic potential. A Salmonella typhimurium reverse mutation assay was executed with strains TA98, TA100, TA1535, TA1537 and TA1538 at a concentration range for TEG of 1–113 mg per plate in the presence and absence of a metabolic activation system. There was no evidence for an increased mutation frequency (Ballantyne and Snelligs, Appl. Toxicol. 27: 291–299, 2007).