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EC number: 240-166-3 | CAS number: 16026-16-7
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 17 May - 14 Jun 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
Further information is included under 'Attached justification' in IUCLID section 13 and 'Cross-reference'. - Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Activated sludge was collected on 13 May 2005 from the municipal waste water treatment plant Neustadt/Weinstrasse, Lachen-Speyerdorf
- Method of cultivation: The sludge was continuously aerated and washed with tap water and OECD medium
- Concentration of suspended solids: 12.78 g/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- other: TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral medium as recommended by the guideline
- Test temperature: 21 ± 1 °C
- Aeration of dilution water: All vessels were aerated overnight with CO2-free air prior to test start and during the test
- Suspended solids concentration: 25 mg/L
TEST SYSTEM
- Culturing apparatus: 2 L Glass bottles, 1500 mL fill volume
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: Aeration with CO2-free air
- Details of trap for CO2: Outgoing air from the test vessels passed two serial bottles containing 0.25 M NaOH solution
- Other:
SAMPLING
- Sampling frequency: Measurement of evolved CO2 on days 2, 4, 6, 8, 10, 14, 21, 28 and 29
- Sampling method: 0.5 mL aliquots of the NaOH solution was sampled at each time point and analysed using a TOC 5050A (Shimadzu)
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 vessels
- Abiotic sterile control: 1 vessel
- Toxicity control: 1 vessel - Reference substance:
- aniline
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 85.2
- Sampling time:
- 28 d
- Details on results:
- - The toxicity control attained 98.3% degradation.
- Abiotic degradation was 26.6%. - Results with reference substance:
- The reference substance attained 79.9% degradation after 28 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 17 May - 14 Jun 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
Further information is included under 'Attached justification' in IUCLID section 13 and 'Cross-reference'. - Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Activated sludge was collected on 13 May 2005 from the municipal waste water treatment plant Neustadt/Weinstrasse, Lachen-Speyerdorf
- Method of cultivation: The sludge was continuously aerated and washed with tap water and OECD medium
- Concentration of suspended solids: 12.78 g/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- other: TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral medium as recommended by the guideline
- Test temperature: 21 ± 1 °C
- Aeration of dilution water: All vessels were aerated overnight with CO2-free air prior to test start and during the test
- Suspended solids concentration: 25 mg/L
TEST SYSTEM
- Culturing apparatus: 2 L Glass bottles, 1500 mL fill volume
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: Aeration with CO2-free air
- Details of trap for CO2: Outgoing air from the test vessels passed two serial bottles containing 0.25 M NaOH solution
- Other:
SAMPLING
- Sampling frequency: Measurement of evolved CO2 on days 2, 4, 6, 8, 10, 14, 21, 28 and 29
- Sampling method: 0.5 mL aliquots of the NaOH solution was sampled at each time point and analysed using a TOC 5050A (Shimadzu)
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 vessels
- Abiotic sterile control: 1 vessel
- Toxicity control: 1 vessel - Reference substance:
- aniline
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 85.2
- Sampling time:
- 28 d
- Details on results:
- - The toxicity control attained 98.3% degradation.
- Abiotic degradation was 26.6%. - Results with reference substance:
- The reference substance attained 79.9% degradation after 28 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
Referenceopen allclose all
Table 1: Percentage of Degradation
Day |
% Degradation |
|||
Reference |
Test Item (mean of duplicates) |
Abiotic Control |
Toxicity Control |
|
2 |
2.6 |
1.7 |
3.4 |
3.2 |
4 |
26.5 |
24.1 |
3.1 |
53.5 |
6 |
56.1 |
47.5 |
11.3 |
68.7 |
8 |
66.5 |
57.4 |
16.0 |
75.8 |
10 |
71.1 |
61.3 |
8.6 |
79.7 |
14 |
76.2 |
72.9 |
17.6 |
86.7 |
21 |
85.0 |
88.3 |
22.9 |
97.5 |
28 |
77.6 |
83.0 |
28.0 |
99.6 |
29 |
79.9 |
85.2 |
26.6 |
98.3 |
Table 1: Percentage of Degradation
Day |
% Degradation |
|||
Reference |
Test Item (mean of duplicates) |
Abiotic Control |
Toxicity Control |
|
2 |
2.6 |
1.7 |
3.4 |
3.2 |
4 |
26.5 |
24.1 |
3.1 |
53.5 |
6 |
56.1 |
47.5 |
11.3 |
68.7 |
8 |
66.5 |
57.4 |
16.0 |
75.8 |
10 |
71.1 |
61.3 |
8.6 |
79.7 |
14 |
76.2 |
72.9 |
17.6 |
86.7 |
21 |
85.0 |
88.3 |
22.9 |
97.5 |
28 |
77.6 |
83.0 |
28.0 |
99.6 |
29 |
79.9 |
85.2 |
26.6 |
98.3 |
Description of key information
Calcium bis[(Z)-N-methyl-N-(1-oxo-9-octadecenyl)aminoacetate] is considered to be readily biodegradable based on a study conducted with a read across substance, where 85.2 % biodegradation was observed after 28 days.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
No biodegradation study is available for the substance calcium bis[(Z)-N-methyl-N-(1-oxo-9-octadecenyl)aminoacetate]. The biodegradation endpoint is completed by reading across data from N-methyl-N-(C18-(unsaturated)alkanoyl)glycine (EC 701-177-3).
The ready biodegradability study was conducted according to the OECD guideline 301B using non-adapted activated sludge from a municipal waste water treatment plant (LAUS 2005). The test item was inoculated with domestic, non-adapted activated sludge for 28 days under aeration. Inoculum blanks, an abiotic sterile control and a toxicity control were run in parallel. After 28 days of incubation, the test item achieved 85.2% degradation based on CO2 evolution, therefore it can be concluded that the test item is readily biodegradable according to the OECD guideline criteria.
The study was conducted in accordance with an OECD test guideline and is GLP-compliant and is therefore considered reliable without restrictions (Klimisch 1).
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